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  <headerData>
    <submissionType>13F-HR</submissionType>
    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <filer>
        <credentials>
          <cik>0001179392</cik>
          <ccc>XXXXXXXX</ccc>
        </credentials>
      </filer>
      <periodOfReport>03-31-2026</periodOfReport>
    </filerInfo>
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  <formData>
    <coverPage>
      <reportCalendarOrQuarter>03-31-2026</reportCalendarOrQuarter>
      <isAmendment>false</isAmendment>
      <filingManager>
        <name>TWO SIGMA INVESTMENTS, LP</name>
        <address>
          <com:street1>100 AVENUE OF THE AMERICAS</com:street1>
          <com:street2>16TH FLOOR</com:street2>
          <com:city>NEW YORK</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10013</com:zipCode>
        </address>
      </filingManager>
      <reportType>13F HOLDINGS REPORT</reportType>
      <form13FFileNumber>028-10385</form13FFileNumber>
      <crdNumber>000137137</crdNumber>
      <secFileNumber>801-70476</secFileNumber>
      <provideInfoForInstruction5>Y</provideInfoForInstruction5>
      <additionalInformation>This Form 13F Holdings Report (the "Report") is being filed on behalf of Two Sigma Investments, LP ("TSI"). TSI serves as the sole manager or investment manager, as applicable, of: (i) Two Sigma Equity Portfolio, LLC, Two Sigma Spectrum Portfolio, LLC, Two Sigma Futures Portfolio, LLC, Two Sigma Horizon Portfolio, LLC , Two Sigma Blazar Portfolio, LLC, Two Sigma Navigator Portfolio, LLC, Two Sigma Equity Spectrum Portfolio, LLC, Two Sigma Venus Portfolio, LLC, Two Sigma Absolute Return Portfolio, LLC, Two Sigma Active Extension U.S. All Cap Equity Portfolio, LLC, Two Sigma Active Extension U.S. Small Cap Equity Fund, LP, Two Sigma World Core Fund, LP and Two Sigma Equity Risk Premia Portfolio, LLC, thirteen investment entities that are managed by TSI, each of which may be deemed to be an "other manager" for purposes of this Report (collectively, the "Other Managers"); (ii) Two Sigma Galileo Portfolio, LLC, Two Sigma Pioneer Portfolio, LLC , Two Sigma Horizon Cayman Portfolio, LLC, Two Sigma International Core Portfolio, LLC and Two Sigma Macro Risk Premia Portfolio, LLC, five investment entities that are managed by TSI and that each may not presently be deemed to be an "other manager" for purposes of this Report (collectively, the "Non-Other Managers"); and (iii) one or more separately managed accounts and sub-advised accounts (collectively, the "Managed Accounts"), each of which may not presently be deemed to be an "other manager" for purposes of this Report. TSI, rather than the Other Managers, Non-Other Managers or the Managed Accounts, exercises sole investment discretion and voting authority over the securities held in the various accounts of the Other Managers, Non-Other Managers and the Managed Accounts.</additionalInformation>
    </coverPage>
    <signatureBlock>
      <name>Steve H. Metzger</name>
      <title>General Counsel</title>
      <phone>212-625-5700</phone>
      <signature>/s/ Steve H. Metzger</signature>
      <city>New York</city>
      <stateOrCountry>NY</stateOrCountry>
      <signatureDate>05-15-2026</signatureDate>
    </signatureBlock>
    <summaryPage>
      <otherIncludedManagersCount>13</otherIncludedManagersCount>
      <tableEntryTotal>4546</tableEntryTotal>
      <tableValueTotal>123930684213</tableValueTotal>
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        <otherManager2>
          <sequenceNumber>1</sequenceNumber>
          <otherManager>
            <cik>0001280134</cik>
            <form13FFileNumber>028-10704</form13FFileNumber>
            <name>TWO SIGMA EQUITY PORTFOLIO, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>2</sequenceNumber>
          <otherManager>
            <cik>0001316047</cik>
            <form13FFileNumber>028-11144</form13FFileNumber>
            <name>TWO SIGMA SPECTRUM PORTFOLIO, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>3</sequenceNumber>
          <otherManager>
            <cik>0001600096</cik>
            <form13FFileNumber>028-16011</form13FFileNumber>
            <name>Two Sigma Futures Portfolio, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>4</sequenceNumber>
          <otherManager>
            <cik>0001453226</cik>
            <form13FFileNumber>028-15356</form13FFileNumber>
            <name>TWO SIGMA HORIZON PORTFOLIO, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>5</sequenceNumber>
          <otherManager>
            <cik>0001802995</cik>
            <form13FFileNumber>028-20138</form13FFileNumber>
            <name>Two Sigma Equity Spectrum Portfolio, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>6</sequenceNumber>
          <otherManager>
            <cik>0001965609</cik>
            <form13FFileNumber>028-23019</form13FFileNumber>
            <name>Two Sigma Blazar Portfolio, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>7</sequenceNumber>
          <otherManager>
            <cik>0001965593</cik>
            <form13FFileNumber>028-23021</form13FFileNumber>
            <name>Two Sigma Navigator Portfolio, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>8</sequenceNumber>
          <otherManager>
            <cik>0002113461</cik>
            <form13FFileNumber>028-26707</form13FFileNumber>
            <name>Two Sigma Venus Portfolio, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>9</sequenceNumber>
          <otherManager>
            <cik>0001569734</cik>
            <form13FFileNumber>028-15367</form13FFileNumber>
            <name>TWO SIGMA ABSOLUTE RETURN PORTFOLIO, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>10</sequenceNumber>
          <otherManager>
            <cik>0001569731</cik>
            <form13FFileNumber>028-15404</form13FFileNumber>
            <name>TWO SIGMA ACTIVE EXTENSION U.S. ALL CAP EQUITY PORTFOLIO, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>11</sequenceNumber>
          <otherManager>
            <cik>0001559804</cik>
            <form13FFileNumber>028-15364</form13FFileNumber>
            <name>Two Sigma Active Extension U.S. Small Cap Equity Fund, LP</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>12</sequenceNumber>
          <otherManager>
            <cik>0001667002</cik>
            <form13FFileNumber>028-17346</form13FFileNumber>
            <name>Two Sigma Equity Risk Premia Portfolio, LLC</name>
          </otherManager>
        </otherManager2>
        <otherManager2>
          <sequenceNumber>13</sequenceNumber>
          <otherManager>
            <cik>0001630305</cik>
            <form13FFileNumber>028-17350</form13FFileNumber>
            <name>Two Sigma World Core Fund, LP</name>
          </otherManager>
        </otherManager2>
      </otherManagers2Info>
    </summaryPage>
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