X0303 4 2010-04-20 0 0001068851 PROSPERITY BANCSHARES INC PRSP 0001136856 HOLMES NED S 55 WUGH DRIVE, SUITE 1111 HOUSTON TX 77027 1 0 0 0 Common Stock 2010-04-20 4 A 0 800 0 A 206705 D Common Stock 99854 I By Profit sharing Plan Common Stock 8820 I As trustee of Grandchildrens' Trust Common Stock 27500 I As Trustee of SSH Trust for Adult Daughter Common Stock 27500 I As Trustee of SSH Trust for Adult Daughter Common Stock 2480 I By Trust Common Stock 2480 I By Trust Common Stock 95070 I By limited partnership Includes 375 shares acquired through the Prosperity Bancshares, Inc. dividend reinvestment plan since the last filing by this reporting person. /s/ Ned S. Holmes 2010-04-22