<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2015-07-15</periodOfReport>

    <notSubjectToSection16>0</notSubjectToSection16>

    <issuer>
        <issuerCik>0001135657</issuerCik>
        <issuerName>Eco-Stim Energy Solutions, Inc.</issuerName>
        <issuerTradingSymbol>ESES</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001385343</rptOwnerCik>
            <rptOwnerName>ACM Emerging Markets Master Fund I L P</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O ALBRIGHT CAPITAL MANAGEMENT LLC</rptOwnerStreet1>
            <rptOwnerStreet2>1101 NEW YORK AVENUE, NW</rptOwnerStreet2>
            <rptOwnerCity>WASHINGTON,</rptOwnerCity>
            <rptOwnerState>DC</rptOwnerState>
            <rptOwnerZipCode>20005</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001621889</rptOwnerCik>
            <rptOwnerName>Albright Capital Management LLC</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>1101 NEW YORK AVENUE</rptOwnerStreet1>
            <rptOwnerStreet2>NW</rptOwnerStreet2>
            <rptOwnerCity>WASHINGTON,</rptOwnerCity>
            <rptOwnerState>DC</rptOwnerState>
            <rptOwnerZipCode>20005</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
            <officerTitle></officerTitle>
            <otherText></otherText>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2015-07-15</value>
            </transactionDate>
            <deemedExecutionDate></deemedExecutionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>J</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionTimeliness></transactionTimeliness>
            <transactionAmounts>
                <transactionShares>
                    <value>523192</value>
                    <footnoteId id="F1"/>
                </transactionShares>
                <transactionPricePerShare>
                    <value>4.75</value>
                    <footnoteId id="F1"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>2030436</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                    <footnoteId id="F2"/>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">Eco-Stim Energy Solutions, Inc. (the &quot;Issuer&quot;) previously entered into a First Amendment to the Convertible Note Facility Agreement (as amended, the &quot;Note Agreement&quot;) with ACM Emerging Markets Master Funds I, L.P. (the &quot;Fund&quot;), pursuant to which the Fund elected to have the interest payable on the Note Agreement on May 28, 2015 (the &quot;Deferred Interest&quot;) paid in the form of shares of the Issuer's Common Stock issuable upon the consummation of a Specified Equity Offering (as defined in the Note Agreement). On July 15, 2015, upon consummation of an underwritten public offering by the Issuer and pursuant to the Note Agreement, Deferred Interest in the amount of $2,485,163 was converted into 523,192 shares of the Issuer's Common Stock at a price of $4.75 per share.</footnote>
        <footnote id="F2">Albright Capital Management LLC (the &quot;GP&quot;), as general partner of the Fund, may be deemed to beneficially own the securities reported herein to the extent of its direct or indirect interest in the Fund. Pursuant to Rule 16a-1(a)(4) under the Securities Act of 1934, as amended (the &quot;Exchange Act&quot;), this filing shall not be deemed an admission that the GP is, for purposes of Section 16 of the Exchange Act or otherwise, the beneficial owner of any securities in excess of its pecuniary interests, if any.</footnote>
    </footnotes>

    <remarks>The Fund and the GP are jointly filing this Form 4 pursuant to Rule 16a-3(j) under the Exchange Act.</remarks>

    <ownerSignature>
        <signatureName>/s/ Gregory Bowes</signatureName>
        <signatureDate>2015-07-16</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Gregory Bowes</signatureName>
        <signatureDate>2015-07-16</signatureDate>
    </ownerSignature>
</ownershipDocument>
