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  <schemaVersion>X0202</schemaVersion>
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    <submissionType>13F-HR</submissionType>
    <filerInfo>
      <liveTestFlag>LIVE</liveTestFlag>
      <filer>
        <credentials>
          <cik>0001132651</cik>
          <ccc>XXXXXXXX</ccc>
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        <fileNumber>028-25350</fileNumber>
      </filer>
      <periodOfReport>06-30-2025</periodOfReport>
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    <coverPage>
      <reportCalendarOrQuarter>06-30-2025</reportCalendarOrQuarter>
      <filingManager>
        <name>AMES NATIONAL CORP</name>
        <address>
          <com:street1>PO BOX 846</com:street1>
          <com:city>AMES</com:city>
          <com:stateOrCountry>IA</com:stateOrCountry>
          <com:zipCode>50010</com:zipCode>
        </address>
      </filingManager>
      <reportType>13F HOLDINGS REPORT</reportType>
      <form13FFileNumber>028-25350</form13FFileNumber>
      <provideInfoForInstruction5>N</provideInfoForInstruction5>
    </coverPage>
    <signatureBlock>
      <name>John P. Nelson</name>
      <title>President/CEO</title>
      <phone>515-620-5917</phone>
      <signature>/s/ John P. Nelson</signature>
      <city>Ames</city>
      <stateOrCountry>IA</stateOrCountry>
      <signatureDate>08-11-2025</signatureDate>
    </signatureBlock>
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      <otherIncludedManagersCount>1</otherIncludedManagersCount>
      <tableEntryTotal>123</tableEntryTotal>
      <tableValueTotal>154641627</tableValueTotal>
      <isConfidentialOmitted>false</isConfidentialOmitted>
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          <sequenceNumber>1</sequenceNumber>
          <otherManager>
            <cik>0002058589</cik>
            <form13FFileNumber>028-25528</form13FFileNumber>
            <name>First National Bank, Ames, Iowa</name>
          </otherManager>
        </otherManager2>
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