<?xml version="1.0" encoding="UTF-8"?><edgarSubmission xmlns="http://www.sec.gov/edgar/ownership" xmlns:com="http://www.sec.gov/edgar/common">
  <headerData>
    <submissionType>144</submissionType>
    <filerInfo>
      <filer>
        <filerCredentials>
          <cik>0001114357</cik>
          <ccc>XXXXXXXX</ccc>
        </filerCredentials>
      </filer>
      <liveTestFlag>LIVE</liveTestFlag>


    </filerInfo>
  </headerData>
  <formData>
    <issuerInfo>
      <issuerCik>0001717115</issuerCik>
      <issuerName>Tempus AI, Inc.</issuerName>
      <secFileNumber>001-42130</secFileNumber>
      <issuerAddress>
        <com:street1>600 WEST CHICAGO AVENUE</com:street1>
        <com:street2>SUITE 510</com:street2>
        <com:city>CHICAGO</com:city>
        <com:stateOrCountry>IL</com:stateOrCountry>
        <com:zipCode>60654</com:zipCode>
      </issuerAddress>
      <issuerContactPhone>800-976-5448</issuerContactPhone>
      <nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>LEFKOFSKY ERIC P</nameOfPersonForWhoseAccountTheSecuritiesAreToBeSold>
      <relationshipsToIssuer>
        <relationshipToIssuer>Officer</relationshipToIssuer>
        <relationshipToIssuer>Director</relationshipToIssuer>
        <relationshipToIssuer>10% Stockholder</relationshipToIssuer>
      </relationshipsToIssuer>
    </issuerInfo>
    <securitiesInformation>
      <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
      <brokerOrMarketmakerDetails>
        <name>Morgan Stanley Smith Barney LLC</name>
        <address>
          <com:street1>1 New York Plaza, 8th Floor</com:street1>
          <com:city>New York</com:city>
          <com:stateOrCountry>NY</com:stateOrCountry>
          <com:zipCode>10004</com:zipCode>
        </address>
      </brokerOrMarketmakerDetails>
      <noOfUnitsSold>520000</noOfUnitsSold>
      <aggregateMarketValue>26530400.00</aggregateMarketValue>
      <noOfUnitsOutstanding>152401894</noOfUnitsOutstanding>
      <approxSaleDate>01/29/2025</approxSaleDate>
      <securitiesExchangeName>Nasdaq</securitiesExchangeName>
    </securitiesInformation>
    <securitiesToBeSold>
      <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
      <acquiredDate>01/29/2025</acquiredDate>
      <natureOfAcquisitionTransaction>Units Vesting Under a Registered Plan - See Remarks</natureOfAcquisitionTransaction>
      <nameOfPersonfromWhomAcquired>Issuer</nameOfPersonfromWhomAcquired>
      <isGiftTransaction>N</isGiftTransaction>
      <amountOfSecuritiesAcquired>520000</amountOfSecuritiesAcquired>
      <paymentDate>01/29/2025</paymentDate>
      <natureOfPayment>N/A</natureOfPayment>
    </securitiesToBeSold>
    <nothingToReportFlagOnSecuritiesSoldInPast3Months>N</nothingToReportFlagOnSecuritiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Eric P. Lefkofsky</name>
        <address>
          <com:street1>c/o Tempus AI, Inc.</com:street1>
          <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
          <com:city>Chicago</com:city>
          <com:stateOrCountry>IL</com:stateOrCountry>
          <com:zipCode>60654</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
      <saleDate>01/24/2025</saleDate>
      <amountOfSecuritiesSold>85788</amountOfSecuritiesSold>
      <grossProceeds>4580521.60</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Eric P. Lefkofsky</name>
        <address>
          <com:street1>c/o Tempus AI, Inc</com:street1>
          <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
          <com:city>Chicago</com:city>
          <com:stateOrCountry>IL</com:stateOrCountry>
          <com:zipCode>60654</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
      <saleDate>01/23/2025</saleDate>
      <amountOfSecuritiesSold>645068</amountOfSecuritiesSold>
      <grossProceeds>33710610.15</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Eric P. Lefkofsky</name>
        <address>
          <com:street1>c/o Tempus AI, Inc.</com:street1>
          <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
          <com:city>Chicago</com:city>
          <com:stateOrCountry>IL</com:stateOrCountry>
          <com:zipCode>60654</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
      <saleDate>01/22/2025</saleDate>
      <amountOfSecuritiesSold>476209</amountOfSecuritiesSold>
      <grossProceeds>23733981.20</grossProceeds>
    </securitiesSoldInPast3Months>
    <securitiesSoldInPast3Months>
      <sellerDetails>
        <name>Eric P. Lefkofsky</name>
        <address>
          <com:street1>c/o Tempus AI, Inc.</com:street1>
          <com:street2>600 West Chicago Avenue, Suite 510</com:street2>
          <com:city>Chicago</com:city>
          <com:stateOrCountry>IL</com:stateOrCountry>
          <com:zipCode>60654</com:zipCode>
        </address>
      </sellerDetails>
      <securitiesClassTitle>Class A Common Stock</securitiesClassTitle>
      <saleDate>12/18/2024</saleDate>
      <amountOfSecuritiesSold>187655</amountOfSecuritiesSold>
      <grossProceeds>7045623.36</grossProceeds>
    </securitiesSoldInPast3Months>
    <remarks>The sales reported on this Form 144 are mandated by the Issuer's election under its equity incentive plan to require the satisfaction of a tax withholding obligation to be funded by sell to cover transactions and do not represent discretionary trades by the reporting person. Amount reported represents the estimated number of shares to be sold in mandatory sell to cover transactions upon the settlement of restricted stock units between January 29, 2025 and February 7, 2025. The actual number of shares sold will depend on the sale prices of the Issuer's Class A common stock in such transactions and the reporting person's personal tax rates.</remarks>
    <noticeSignature>
      <noticeDate>01/29/2025</noticeDate>
      <signature>/s/ Eric P. Lefkofsky</signature>
    </noticeSignature>
  </formData>
</edgarSubmission>
