<?xml version="1.0"?>
<edgarSubmission>

    <schemaVersion>X0708</schemaVersion>

    <submissionType>D</submissionType>

    <testOrLive>LIVE</testOrLive>

    <primaryIssuer>
        <cik>0001113423</cik>
        <entityName>Aeterna Zentaris Inc.</entityName>
        <issuerAddress>
            <street1>315 SIGMA DRIVE</street1>
            <city>SUMMERVILLE</city>
            <stateOrCountry>SC</stateOrCountry>
            <stateOrCountryDescription>SOUTH CAROLINA</stateOrCountryDescription>
            <zipCode>29486</zipCode>
        </issuerAddress>
        <issuerPhoneNumber>843-900-3201</issuerPhoneNumber>
        <jurisdictionOfInc>CANADA (FEDERAL LEVEL)</jurisdictionOfInc>
        <issuerPreviousNameList>
            <previousName>Aeterna Laboratories Inc.</previousName>
        </issuerPreviousNameList>
        <edgarPreviousNameList>
            <previousName>AETERNA LABORATORIES INC</previousName>
        </edgarPreviousNameList>
        <entityType>Corporation</entityType>
        <yearOfInc>
            <overFiveYears>true</overFiveYears>
        </yearOfInc>
    </primaryIssuer>

    <relatedPersonsList>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Michael</firstName>
                <middleName>V.</middleName>
                <lastName>Ward</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>315 Sigma Drive</street1>
                <city>Summerville</city>
                <stateOrCountry>SC</stateOrCountry>
                <stateOrCountryDescription>SOUTH CAROLINA</stateOrCountryDescription>
                <zipCode>29486</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Leslie</firstName>
                <lastName>Auld</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>315 Sigma Drive</street1>
                <city>Summerville</city>
                <stateOrCountry>SC</stateOrCountry>
                <stateOrCountryDescription>SOUTH CAROLINA</stateOrCountryDescription>
                <zipCode>29486</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Klaus</firstName>
                <lastName>Paulini</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Weismullerstrasse 50</street1>
                <city>Frankfurt</city>
                <stateOrCountry>2M</stateOrCountry>
                <stateOrCountryDescription>GERMANY</stateOrCountryDescription>
                <zipCode>60314</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Nicola</firstName>
                <lastName>Ammer</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Weismullerstrasse 50</street1>
                <city>Frankfurt</city>
                <stateOrCountry>2M</stateOrCountry>
                <stateOrCountryDescription>GERMANY</stateOrCountryDescription>
                <zipCode>60314</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Executive Officer</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Carolyn</firstName>
                <lastName>Egbert</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>315 Sigma Drive</street1>
                <city>Summerville</city>
                <stateOrCountry>SC</stateOrCountry>
                <stateOrCountryDescription>SOUTH CAROLINA</stateOrCountryDescription>
                <zipCode>29486</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Robin</firstName>
                <lastName>Smith Hoke</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>315 Sigma Drive</street1>
                <city>Summerville</city>
                <stateOrCountry>SC</stateOrCountry>
                <stateOrCountryDescription>SOUTH CAROLINA</stateOrCountryDescription>
                <zipCode>29486</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Gerard</firstName>
                <lastName>Limoges</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>Weismullerstrasse 50</street1>
                <city>Frankfurt</city>
                <stateOrCountry>2M</stateOrCountry>
                <stateOrCountryDescription>GERMANY</stateOrCountryDescription>
                <zipCode>60314</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
        <relatedPersonInfo>
            <relatedPersonName>
                <firstName>Brent</firstName>
                <lastName>Norton</lastName>
            </relatedPersonName>
            <relatedPersonAddress>
                <street1>315 Sigma Drive</street1>
                <city>Summerville</city>
                <stateOrCountry>SC</stateOrCountry>
                <stateOrCountryDescription>SOUTH CAROLINA</stateOrCountryDescription>
                <zipCode>29486</zipCode>
            </relatedPersonAddress>
            <relatedPersonRelationshipList>
                <relationship>Director</relationship>
            </relatedPersonRelationshipList>
        </relatedPersonInfo>
    </relatedPersonsList>

    <offeringData>
        <industryGroup>
            <industryGroupType>Pharmaceuticals</industryGroupType>
        </industryGroup>
        <issuerSize>
            <revenueRange>Decline to Disclose</revenueRange>
        </issuerSize>
        <federalExemptionsExclusions>
            <item>06b</item>
        </federalExemptionsExclusions>
        <typeOfFiling>
            <newOrAmendment>
                <isAmendment>false</isAmendment>
            </newOrAmendment>
            <dateOfFirstSale>
                <value>2019-09-20</value>
            </dateOfFirstSale>
        </typeOfFiling>
        <durationOfOffering>
            <moreThanOneYear>false</moreThanOneYear>
        </durationOfOffering>
        <typesOfSecuritiesOffered>
            <isOptionToAcquireType>true</isOptionToAcquireType>
            <isSecurityToBeAcquiredType>true</isSecurityToBeAcquiredType>
        </typesOfSecuritiesOffered>
        <businessCombinationTransaction>
            <isBusinessCombinationTransaction>false</isBusinessCombinationTransaction>
        </businessCombinationTransaction>
        <minimumInvestmentAccepted>0</minimumInvestmentAccepted>
        <salesCompensationList>
            <recipient>
                <recipientName>Maxim Group LLC</recipientName>
                <recipientCRDNumber>120708</recipientCRDNumber>
                <associatedBDName>None</associatedBDName>
                <associatedBDCRDNumber>None</associatedBDCRDNumber>
                <recipientAddress>
                    <street1>405 LEXINGTON AVENUE</street1>
                    <city>NEW YORK</city>
                    <stateOrCountry>NY</stateOrCountry>
                    <stateOrCountryDescription>NEW YORK</stateOrCountryDescription>
                    <zipCode>10174</zipCode>
                </recipientAddress>
                <statesOfSolicitationList>
                    <state>NY</state>
                    <description>NEW YORK</description>
                </statesOfSolicitationList>
                <foreignSolicitation>false</foreignSolicitation>
            </recipient>
        </salesCompensationList>
        <offeringSalesAmounts>
            <totalOfferingAmount>4987500</totalOfferingAmount>
            <totalAmountSold>4987500</totalAmountSold>
            <totalRemaining>0</totalRemaining>
            <clarificationOfResponse>The issuer received gross proceeds of $4,987,500 from the issuance of 3,325,000 common shares in a registered direct  offering and a concurrent private placement of warrants to purchase up to 3,325,000 common shares.</clarificationOfResponse>
        </offeringSalesAmounts>
        <investors>
            <hasNonAccreditedInvestors>false</hasNonAccreditedInvestors>
            <totalNumberAlreadyInvested>4</totalNumberAlreadyInvested>
        </investors>
        <salesCommissionsFindersFees>
            <salesCommissions>
                <dollarAmount>349125</dollarAmount>
            </salesCommissions>
            <findersFees>
                <dollarAmount>0</dollarAmount>
            </findersFees>
            <clarificationOfResponse>The commissions were based on gross proceeds of the registered direct  offering of common shares that was concurrent with the private placement  of unregistered warrants.</clarificationOfResponse>
        </salesCommissionsFindersFees>
        <useOfProceeds>
            <grossProceedsUsed>
                <dollarAmount>0</dollarAmount>
            </grossProceedsUsed>
            <clarificationOfResponse></clarificationOfResponse>
        </useOfProceeds>
        <signatureBlock>
            <authorizedRepresentative>false</authorizedRepresentative>
            <signature>
                <issuerName>Aeterna Zentaris Inc.</issuerName>
                <signatureName>/s/ Michael V. Ward</signatureName>
                <nameOfSigner>Michael V. Ward</nameOfSigner>
                <signatureTitle>President and CEO</signatureTitle>
                <signatureDate>2019-10-01</signatureDate>
            </signature>
        </signatureBlock>
    </offeringData>
</edgarSubmission>
