X0202 SCHEDULE 13G/A 0001103804 XXXXXXXX LIVE 4 Class A Common Stock, par value $0.0001 per share 03/31/2026 0001830214 Ginkgo Bioworks Holdings, Inc. 37611X209 27 Drydock Avenue 8th Floor Boston MA 02210 Rule 13d-1(c) VIKING GLOBAL INVESTORS LP b DE 0 3577128 0 3577128 3577128 N 7.1 PN See Item 4 Viking Global Opportunities Parent GP LLC b DE 0 3577128 0 3577128 3577128 N 7.1 OO See Item 4 Viking Global Opportunities GP LLC b DE 0 3577128 0 3577128 3577128 N 7.1 OO See Item 4 Viking Global Opportunities Portfolio GP LLC b DE 0 3577128 0 3577128 3577128 N 7.1 OO See Item 4 Viking Global Opportunities Illiquid Investments Sub-Master LP b E9 0 3577128 0 3577128 3577128 N 7.1 PN See Item 4 HALVORSEN OLE ANDREAS b Q8 0 3577128 0 3577128 3577128 N 7.1 IN See Item 4 Shabet Rose Sharon b X1 0 3577128 0 3577128 3577128 N 7.1 IN See Item 4 Ginkgo Bioworks Holdings, Inc. 27 Drydock Avenue, 8th Floor, Boston, MA 02210 Viking Global Investors LP ("VGI"), Viking Global Opportunities Parent GP LLC ("Opportunities Parent"), Viking Global Opportunities GP LLC ("Opportunities GP"), Viking Global Opportunities Portfolio GP LLC ("Opportunities Portfolio GP"), Viking Global Opportunities Illiquid Investments Sub-Master LP ("VGOP"), O. Andreas Halvorsen and Rose S. Shabet (collectively, the "Reporting Persons") Effective March 31, 2026, David C. Ott ("Mr. Ott") retired from his roles as Advisory Director of VGI and Executive Committee Member of each of Viking Global Partners LLC (the general partner of VGI) and Opportunities Parent. Accordingly, this Amendment No. 4 is being filed to remove Mr. Ott as a Reporting Person from this Schedule 13G, as Mr. Ott is no longer a beneficial owner of any of the shares of Class A Common Stock reported herein. The business address of each of the Reporting Persons is: 600 Washington Boulevard, Floor 11, Stamford, Connecticut 06901. VGI is a Delaware limited partnership; Opportunities Parent, Opportunities GP and Opportunities Portfolio GP are Delaware limited liability companies; VGOP is a Cayman Islands exempted limited partnership; O. Andreas Halvorsen is a citizen of Norway; and Rose S. Shabet is a citizen of the United States. Y VGI: 3,577,128 VGI provides managerial services to VGOP. VGI has the authority to dispose of and vote the shares of Class A Common Stock directly held by VGOP. VGI does not directly own any shares of Class A Common Stock. Based on Rule 13d-3 under the Securities Exchange Act of 1934, as amended (the "Act"), VGI may be deemed to beneficially own the shares of Class A Common Stock directly held by VGOP. VGI beneficially owns 3,577,128 shares of Class A Common Stock consisting of (i) 2,900,025 shares of Class A Common Stock directly and beneficially owned by VGOP and (ii) 677,103 shares of Class A Common Stock directly and beneficially owned by VGOP that remain subject to forfeiture to the extent certain price targets are not satisfied. Opportunities Parent: 3,577,128 Opportunities Parent is the sole member of Opportunities GP, which has the authority to dispose of and vote the shares of Class A Common Stock controlled by Opportunities Portfolio GP, which consists of the shares of Class A Common Stock directly held by VGOP. Opportunities Parent does not directly own any shares of Class A Common Stock. Based on Rule 13d-3 under the Act, Opportunities Parent may be deemed to beneficially own the shares of Class A Common Stock directly held by VGOP. Opportunities Parent beneficially owns 3,577,128 shares of Class A Common Stock consisting of (i) 2,900,025 shares of Class A Common Stock directly and beneficially owned by VGOP and (ii) 677,103 shares of Class A Common Stock directly and beneficially owned by VGOP that remain subject to forfeiture to the extent certain price targets are not satisfied. Opportunities GP: 3,577,128 Opportunities GP serves as the sole member of Opportunities Portfolio GP and has the authority to dispose of and vote the shares of Class A Common Stock controlled by Opportunities Portfolio GP, which consists of the shares of Class A Common Stock directly held by VGOP. Opportunities GP does not directly own any shares of Class A Common Stock. Based on Rule 13d-3 under the Act, Opportunities GP may be deemed to beneficially own the shares of Class A Common Stock directly held by VGOP. Opportunities GP beneficially owns 3,577,128 shares of Class A Common Stock consisting of (i) 2,900,025 shares of Class A Common Stock directly and beneficially owned by VGOP and (ii) 677,103 shares of Class A Common Stock directly and beneficially owned by VGOP that remain subject to forfeiture to the extent certain price targets are not satisfied. Opportunities Portfolio GP: 3,577,128 Opportunities Portfolio GP serves as the general partner of VGOP and has the authority to dispose of and vote the shares of Class A Common Stock directly owned by VGOP. Opportunities Portfolio GP does not directly own any shares of Class A Common Stock. Based on Rule 13d-3 under the Act, Opportunities Portfolio GP may be deemed to beneficially own the shares of Class A Common Stock directly held by VGOP. Opportunities Portfolio GP beneficially owns 3,577,128 shares of Class A Common Stock consisting of (i) 2,900,025 shares of Class A Common Stock directly and beneficially owned by VGOP and (ii) 677,103 shares of Class A Common Stock directly and beneficially owned by VGOP that remain subject to forfeiture to the extent certain price targets are not satisfied. VGOP: 3,577,128 VGOP has the authority to dispose of and vote the shares of Class A Common Stock directly owned by it, which power may be exercised by its general partner, Opportunities Portfolio GP, and by VGI, an affiliate of Opportunities Portfolio GP, which provides managerial services to VGOP. Viking Global Opportunities LP (a Delaware limited partnership) and Viking Global Opportunities III LP (a Cayman Islands exempted limited partnership), through its investment in Viking Global Opportunities Intermediate LP (a Cayman Islands exempted limited partnership), invest substantially all of their assets in Viking Global Opportunities Master LP (a Cayman Islands exempted limited partnership), which in turn invests through VGOP. VGOP directly and beneficially owns 3,577,128 shares of Class A Common Stock consisting of (i) 2,900,025 shares of Class A Common Stock directly and beneficially owned by VGOP and (ii) 677,103 shares of Class A Common Stock directly and beneficially owned by VGOP that remain subject to forfeiture to the extent certain price targets are not satisfied. O. Andreas Halvorsen and Rose S. Shabet: 3,577,128 Mr. Halvorsen and Ms. Shabet, as Executive Committee Members of Viking Global Partners LLC (general partner of VGI) and Opportunities Parent, have shared authority to dispose of and vote the shares of Class A Common Stock beneficially owned by VGI and Opportunities Parent. Neither Mr. Halvorsen nor Ms. Shabet directly owns any shares of Class A Common Stock. Based on Rule 13d-3 under the Act, each may be deemed to beneficially own the shares of Class A Common Stock directly held by VGOP. Mr. Halvorsen and Ms. Shabet each beneficially own 3,577,128 shares of Class A Common Stock consisting of (i) 2,900,025 shares of Class A Common Stock directly and beneficially owned by VGOP and (ii) 677,103 shares of Class A Common Stock directly and beneficially owned by VGOP that remain subject to forfeiture to the extent certain price targets are not satisfied. The percentages set forth herein are based on 50,254,065 shares of Class A Common Stock outstanding as of March 31, 2026, as reported in the Issuer's quarterly report on Form 10-Q, filed with the Securities and Exchange Commission (the "Commission") on May 7, 2026. VGI: 7.1% Opportunities Parent: 7.1% Opportunities GP: 7.1% Opportunities Portfolio GP: 7.1% VGOP: 7.1% O. Andreas Halvorsen and Rose S. Shabet: 7.1% VGI: 0 Opportunities Parent: 0 Opportunities GP: 0 Opportunities Portfolio GP: 0 VGOP: 0 O. Andreas Halvorsen and Rose S. Shabet: 0 VGI: 3,577,128 Opportunities Parent: 3,577,128 Opportunities GP: 3,577,128 Opportunities Portfolio GP: 3,577,128 VGOP: 3,577,128 O. Andreas Halvorsen and Rose S. Shabet: 3,577,128 VGI: 0 Opportunities Parent: 0 Opportunities GP: 0 Opportunities Portfolio GP: 0 VGOP: 0 O. Andreas Halvorsen and Rose S. Shabet: 0 VGI: 3,577,128 Opportunities Parent: 3,577,128 Opportunities GP: 3,577,128 Opportunities Portfolio GP: 3,577,128 VGOP: 3,577,128 O. Andreas Halvorsen and Rose S. Shabet: 3,577,128 Y N The response to Item 4 is incorporated by reference herein. Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. EXHIBIT A - JOINT FILING AGREEMENT VIKING GLOBAL INVESTORS LP /s/ Scott M. Hendler 05/15/2026 Viking Global Opportunities Parent GP LLC /s/ Scott M. Hendler 05/15/2026 Viking Global Opportunities GP LLC /s/ Scott M. Hendler 05/15/2026 Viking Global Opportunities Portfolio GP LLC /s/ Scott M. Hendler 05/15/2026 Viking Global Opportunities Illiquid Investments Sub-Master LP /s/ Scott M. Hendler 05/15/2026 HALVORSEN OLE ANDREAS /s/ Scott M. Hendler 05/15/2026 Shabet Rose Sharon /s/ Scott M. Hendler 05/15/2026 (1) Scott M. Hendler is signing on behalf of O. Andreas Halvorsen, individually and as an Executive Committee Member of VIKING GLOBAL PARTNERS LLC, on behalf of VIKING GLOBAL INVESTORS LP, and as an Executive Committee Member of VIKING GLOBAL OPPORTUNITIES PARENT GP LLC, on behalf of itself and VIKING GLOBAL OPPORTUNITIES GP LLC, VIKING GLOBAL OPPORTUNITIES PORTFOLIO GP LLC, and VIKING GLOBAL OPPORTUNITIES ILLIQUID INVESTMENTS SUB-MASTER LP, pursuant to an authorization and designation letter dated February 9, 2021, which was previously filed with the Commission as an exhibit to a Form 13G filed by Mr. Halvorsen on February 12, 2021 (SEC File No. 005-49737). (2) Scott M. Hendler is signing on behalf of Rose S. Shabet, individually and as an Executive Committee Member of VIKING GLOBAL PARTNERS LLC, on behalf of VIKING GLOBAL INVESTORS LP, and as an Executive Committee Member of VIKING GLOBAL OPPORTUNITIES PARENT GP LLC, on behalf of itself and VIKING GLOBAL OPPORTUNITIES GP LLC, VIKING GLOBAL OPPORTUNITIES PORTFOLIO GP LLC, and VIKING GLOBAL OPPORTUNITIES ILLIQUID INVESTMENTS SUB-MASTER LP, pursuant to an authorization and designation letter dated February 9, 2021, which was previously filed with the Commission as an exhibit to a Form 13G filed by Ms. Shabet on February 12, 2021 (SEC File No. 005-49737).