X0201 4 2004-04-01 0 0001115143 ACUSPHERE INC acus 0001134392 BANK OF AMERICA VENTURES 950 TOWER LANE, SUITE 700 FOSTER CITY CA 94404 0 0 1 0 0000070858 BANK OF AMERICA CORP /DE/ BANK OF AMERICA CORPORATE CENTER 100 N TRYON ST CHARLOTTE NC 28255 0 0 1 0 0001102113 BANK OF AMERICA NA 110 N TYRONE CHARLOTTE NC 28255 0 0 1 0 0001014768 NB HOLDINGS CORP 100 NORTH TRYON STREET CHARLOTTE NC 28255 0 0 1 0 Common stock 1314146 D Common stock 146016 I By Banc of America Partners II Common stock 2004-04-01 4 J 0 452414 0 A 452414 I By BancBoston Ventures Inc. Common stock 2004-04-05 4 J 0 6521 0 A 6521 I By Bank of America Capital Corporation Held by BA Venture Partners II, with which the Reporting Persons have an employment releationship. The Reporting Persons disclaim beneficial ownership of such securities. On April 1, 2004, Bank of America Corporation, one of the Reporting Persons, merged with FleetBoston Financial Corporation. As a result of such merger, Bank of America corporation may be deemed to have a pecuniary interest in shares of the Issuer held by BancBoston Ventures, Inc., an indirect wholly-owned subsidiary of FleetBoston Financial Corporation. The Reporting Persons disclaim beneficial ownership of such securities. Held by BancBoston Ventures, Inc. The Reporting Persons disclaim beneficial ownership of such securities. On April 5, 2004, these securities were distributed to Bank of America Capital Corporation, an affiliate of Reporting Persons. Such shares were distributed (for no additional consideration) to Bank of America Capital Corporation by the general partner of a limited partnership of which Bank of America Capital Corporation is a limited partner. Held by Bank of America Capital Corporation. The Reporting Persons disclaim beneficial ownership of such securities. /s/ Kate Mitchell, By: Kate Mitchell, President and Managing Director, Bank of America Ventures 2004-04-05 /s/ Charles S. Bowman, By: Charles S. Bowman, Senior Vice President, Bank of America Corporation 2004-04-05 /s/ Charles S. Bowman, By: Charles S. Bowman, Senior Vice President, Bank of America, N.A. 2004-04-05 /s/ Charles S. Bowman, By: Charles S. Bowman, Senior Vice President, NB Holdings Corporation 2004-04-05