<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3/A</documentType>

    <periodOfReport>2023-10-18</periodOfReport>

    <dateOfOriginalSubmission>2023-11-01</dateOfOriginalSubmission>

    <noSecuritiesOwned>1</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001100397</issuerCik>
        <issuerName>Ayala Pharmaceuticals, Inc.</issuerName>
        <issuerTradingSymbol>ADXS</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001900243</rptOwnerCik>
            <rptOwnerName>Orbach Pini</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>9 DEER PARK DRIVE, SUITE K-1</rptOwnerStreet1>
            <rptOwnerStreet2></rptOwnerStreet2>
            <rptOwnerCity>MONMOUTH JUNCTION</rptOwnerCity>
            <rptOwnerState>NJ</rptOwnerState>
            <rptOwnerZipCode>08852</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>true</isDirector>
        </reportingOwnerRelationship>
    </reportingOwner>

    <remarks>Exhibit 24 - Power of Attorney</remarks>

    <ownerSignature>
        <signatureName>/s/ Roy Golan, as attorney-in-fact for the Reporting Person</signatureName>
        <signatureDate>2023-11-03</signatureDate>
    </ownerSignature>
</ownershipDocument>
