<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2022-01-05</periodOfReport>

    <noSecuritiesOwned>1</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001092662</issuerCik>
        <issuerName>CHEMBIO DIAGNOSTICS, INC.</issuerName>
        <issuerTradingSymbol>CEMI</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001902795</rptOwnerCik>
            <rptOwnerName>Steenvoorden Lawrence J.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O CHEMBIO DIAGNOSTICS, INC.</rptOwnerStreet1>
            <rptOwnerStreet2>555 WIRELESS BOULEVARD</rptOwnerStreet2>
            <rptOwnerCity>HAUPPAUGE</rptOwnerCity>
            <rptOwnerState>NY</rptOwnerState>
            <rptOwnerZipCode>11788</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>true</isOfficer>
            <officerTitle>See remarks</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <remarks>Executive Vice President and Chief Financial Officer. Exhibit List: Exhibit 24 - Power of Attorney</remarks>

    <ownerSignature>
        <signatureName>/s/ Mark L. Johnson, Attorney-in-Fact for Lawrence J. Steenvoorden</signatureName>
        <signatureDate>2022-01-06</signatureDate>
    </ownerSignature>
</ownershipDocument>
