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<!--Created: Thu Mar 14 13:47:25 UTC 2024-->
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      <liveTestFlag>LIVE</liveTestFlag>
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        <issuerCredentials>
          <cik>0001077130</cik>
          <ccc>XXXXXXXX</ccc>
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      <investmentCompanyType>N-6</investmentCompanyType>
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    <generalInfo reportEndingPeriod="2023-12-31" isReportPeriodLt12="N"/>
    <registrantInfo>
      <registrantFullName>COLUMBUS LIFE INSURANCE Co SEPARATE ACCOUNT 1</registrantFullName>
      <investmentCompFileNo>811-09337</investmentCompFileNo>
      <registrantCik>0001077130</registrantCik>
      <registrantLei>54930057CCDHSBCZQM33</registrantLei>
      <registrantstreet1>400 Broadway Street, MS 24</registrantstreet1>
      <registrantcity>Cincinnati</registrantcity>
      <registrantzipCode>45202</registrantzipCode>
      <registrantstate>US-OH</registrantstate>
      <registrantcountry>US</registrantcountry>
      <registrantphoneNumber>513 629 1800</registrantphoneNumber>
      <websites>
        <website webpage="https://www.westernsouthern.com"/>
      </websites>
      <locationBooksRecords>
        <locationBooksRecord>
          <officeName>Jason Nickles</officeName>
          <officeAddress1>400 Broadway Street, MS 24</officeAddress1>
          <officeCity>Cincinnati</officeCity>
          <officeStateCountry officeState="US-OH" officeCountry="US"/>
          <officeRecordsZipCode>45202</officeRecordsZipCode>
          <officePhone>513 629 7418</officePhone>
          <booksRecordsDesc>Charter, By-Laws, Board Minutes., Trial Balance(TB), TB Detail.</booksRecordsDesc>
        </locationBooksRecord>
      </locationBooksRecords>
      <isRegistrantFirstFiling>N</isRegistrantFirstFiling>
      <isRegistrantLastFiling>N</isRegistrantLastFiling>
      <registrantFamilyInvComp isRegistrantFamilyInvComp="Y" familyInvCompFullName="WESTERN &amp; SOUTHERN FINANCIAL GROUP, INC."/>
      <registrantClassificationType>N-6</registrantClassificationType>
      <isSecuritiesActRegistration>Y</isSecuritiesActRegistration>
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        <chiefComplianceOfficer>
          <ccoName>Bruce Maisel</ccoName>
          <crdNumber>001092397</crdNumber>
          <ccoStreet1>400 Broadway</ccoStreet1>
          <ccoCity>Cincinnati</ccoCity>
          <ccoStateCountry ccoState="US-OH" ccoCountry="US"/>
          <ccoZipCode>45202</ccoZipCode>
          <ccoPhone>XXXXXX</ccoPhone>
          <isCcoChangedSinceLastFiling>N</isCcoChangedSinceLastFiling>
          <ccoEmployers>
            <ccoEmployer ccoEmployerName="N/A" ccoEmployerId="N/A"/>
          </ccoEmployers>
        </chiefComplianceOfficer>
      </chiefComplianceOfficers>
      <isRegistrantSubmittedMatter>N</isRegistrantSubmittedMatter>
      <isPreviousLegalProceeding>N</isPreviousLegalProceeding>
      <isPreviousProceedingTerminated>N</isPreviousProceedingTerminated>
      <isFinancialSupportDuringPeriod>N</isFinancialSupportDuringPeriod>
      <isExemptionFromAct>N</isExemptionFromAct>
      <principalUnderwriters>
        <principalUnderwriter>
          <principalUnderwriterName>Touchstone Securities, Inc.</principalUnderwriterName>
          <principalUnderwriterFileNumber>8-14052</principalUnderwriterFileNumber>
          <principalUnderwriterCrdNumber>000001526</principalUnderwriterCrdNumber>
          <principalUnderwriterLei>N/A</principalUnderwriterLei>
          <principalUnderWriterStateCountry principalUnderWriterState="US-NE" principalUnderWriterCountry="US"/>
          <isPrincipalUnderwriterAffiliatedWithRegistrant>Y</isPrincipalUnderwriterAffiliatedWithRegistrant>
        </principalUnderwriter>
      </principalUnderwriters>
      <isUnderwriterHiredOrTerminated>N</isUnderwriterHiredOrTerminated>
      <publicAccountants>
        <publicAccountant>
          <publicAccountantName>ERNST &amp; YOUNG</publicAccountantName>
          <pcaobNumber>(US) 42</pcaobNumber>
          <publicAccountantLei>N/A</publicAccountantLei>
          <publicAccountantStateCountry publicAccountantState="US-DE" publicAccountantCountry="US"/>
        </publicAccountant>
      </publicAccountants>
      <isPublicAccountantChanged>N</isPublicAccountantChanged>
      <isOpinionOffered>N</isOpinionOffered>
      <isMaterialChange>N</isMaterialChange>
      <isAccountingPrincipleChange>N</isAccountingPrincipleChange>
    </registrantInfo>
    <unitInvestmentTrust>
      <depositors>
        <depositor>
          <depositorName>Columbus Life Insurance Company</depositorName>
          <depositorCrdNo>N/A</depositorCrdNo>
          <depositorLei>549300G3M5SISBS7UM12</depositorLei>
          <depositorStateCountry depositorState="US-OH" depositorCountry="US"/>
          <depositorUltimateParentname>WESTERN &amp; SOUTHERN FINANCIAL GROUP , Inc.</depositorUltimateParentname>
        </depositor>
      </depositors>
      <isUitAdminHiredTerminated>N</isUitAdminHiredTerminated>
      <registrantSeparateInsuranceAccount isRegistrantSeparateInsuranceAccount="Y" separateAccountSeriesId="S000000889"/>
      <numOfContracts>812</numOfContracts>
      <contractSecurities>
        <contractSecurity separateAccountSecurityName="Legacy ( Refer to Record 4)" separateAccountContractId="C000002526" separateAccountTotalAsset="0.00" numContractsSold="0" grossPremiumReceived="0.00" grossPremiumReceivedSection1035="0.00" numContractsAffected="0" contractValueRedeemed="0.00" contractValueRedeemedSection1035="0.00" numContractsAffectedRedeemed="0"/>
        <contractSecurity separateAccountSecurityName="Pinnacle ( Refer to Record 3)" separateAccountContractId="C000002527" separateAccountTotalAsset="0.00" numContractsSold="0" grossPremiumReceived="0.00" grossPremiumReceivedSection1035="0.00" numContractsAffected="0" contractValueRedeemed="0.00" contractValueRedeemedSection1035="0.00" numContractsAffectedRedeemed="0"/>
        <contractSecurity separateAccountSecurityName="Pinnacle II" separateAccountContractId="C000002528" separateAccountTotalAsset="42099969.51" numContractsSold="0" grossPremiumReceived="1064596.86" grossPremiumReceivedSection1035="0.00" numContractsAffected="0" contractValueRedeemed="820551.95" contractValueRedeemedSection1035="3638.45" numContractsAffectedRedeemed="2"/>
        <contractSecurity separateAccountSecurityName="Columbus Life Variable Universal Life" separateAccountContractId="C000002529" separateAccountTotalAsset="2522666.12" numContractsSold="0" grossPremiumReceived="116724.69" grossPremiumReceivedSection1035="0.00" numContractsAffected="0" contractValueRedeemed="98637.04" contractValueRedeemedSection1035="0.00" numContractsAffectedRedeemed="0"/>
      </contractSecurities>
      <isRule6C7Reliance>N</isRule6C7Reliance>
      <isRule11A2Reliance>N</isRule11A2Reliance>
      <isRule12D1Dash4Reliance>N</isRule12D1Dash4Reliance>
      <isRule12D1GReliance>N</isRule12D1GReliance>
    </unitInvestmentTrust>
    <attachmentsTab>
      <isLegalProceedings>false</isLegalProceedings>
      <isProvisionFinancialSupport>false</isProvisionFinancialSupport>
      <isIPAReportInternalControl>false</isIPAReportInternalControl>
      <isInfoRequiredEO>false</isInfoRequiredEO>
      <isOtherInfoRequired>false</isOtherInfoRequired>
    </attachmentsTab>
    <signature registrantSignedName="COLUMBUS LIFE INSURANCE Co SEPARATE ACCOUNT 1" signedDate="2024-03-14" signature="Wade M Fugate" title="Vice President and Controller"/>
  </formData>
</edgarSubmission>
