SCHEDULE 13G/A 0001104659-21-119285 0001052192 XXXXXXXX LIVE 1 Class A Common Stock, $0.0001 par value per share 09/30/2025 0001830214 Ginkgo Bioworks Holdings, Inc. 37611X209 27 Drydock Avenue 8th Floor Boston MA 02210 Rule 13d-1(c) Cascade Investment, L.L.C. WA 3621636.00 0.00 3621636.00 0.00 3621636.00 N 7.5 OO All shares of the Class A common stock, $0.0001 par value per share (the "Common Stock") of Ginkgo Bioworks Holdings, Inc. (the "Issuer") held by Cascade Investment, L.L.C. ("Cascade") may be deemed to be beneficially owned by William H. Gates III as the sole member of Cascade. The number of shares beneficially owned includes 299,052 earn-out shares that will vest in substantially equal installments if the Issuer's Common Stock achieves a price per share for any period of 20 trading days out of 30 consecutive trading days prior to September 16, 2026 that equals or exceeds certain thresholds as set forth in the Merger Agreement, dated as of May 11, 2021, by and among Soaring Eagle Acquisition Corp., SEAC Merger Sub Inc. and Ginkgo Bioworks, Inc., as amended (the "Merger Agreement"). Item 11 is based on 48,523,595 shares of Common Stock outstanding as of October 31, 2025, as reported on the Issuer's Form 10-Q filed on November 6, 2025. William H. Gates III X1 3621636.00 0.00 3621636.00 0.00 3621636.00 N 7.5 IN All shares of the Common Stock of the Issuer held by Cascade may be deemed to be beneficially owned by William H. Gates III as the sole member of Cascade. The number of shares beneficially owned includes 299,052 earn-out shares that will vest in substantially equal installments if the Issuer's Common Stock achieves a price per share for any period of 20 trading days out of 30 consecutive trading days prior to September 16, 2026 that equals or exceeds certain thresholds as set forth in the Merger Agreement. Item 11 is based on 48,523,595 shares of Common Stock outstanding as of October 31, 2025, as reported on the Issuer's Form 10-Q filed on November 6, 2025. Ginkgo Bioworks Holdings, Inc. 27 Drydock Avenue 8th Floor Boston, MA, 02210 Cascade Investment, L.L.C. ("Cascade") and William H. Gates III ("WHG"). Cascade - 2365 Carillon Point, Kirkland, Washington 98033 WHG - 500 Fifth Avenue North, Seattle, Washington 98109 Cascade is a limited liability company organized under the laws of the State of Washington. WHG is a citizen of the United States of America. Y See the responses to Item 9 on the attached cover pages. 7.5 See the responses to Item 5 on the attached cover pages. See the responses to Item 6 on the attached cover pages. See the responses to Item 7 on the attached cover pages. See the responses to Item 8 on the attached cover pages. Y Y Y Y Y N By signing below I certify that, to the best of my knowledge and belief, the securities referred to above were not acquired and are not held for the purpose of or with the effect of changing or influencing the control of the issuer of the securities and were not acquired and are not held in connection with or as a participant in any transaction having that purpose or effect, other than activities solely in connection with a nomination under ?? 240.14a-11. Cascade Investment, L.L.C. /s/ Alan Heuberger 11/14/2025 William H. Gates III /s/ Alan Heuberger 11/14/2025 This Amendment is being filed jointly by the Reporting Persons pursuant to the Joint Filing Agreement dated September 24, 2021, and included with the signature page to the Reporting Persons' Schedule 13G with respect to the Issuer filed on September 24, 2021, SEC File No. 005-92710, and incorporated by reference herein. Duly authorized under Special Limited Power of Attorney appointing Alan Heuberger attorney-in-fact, dated October 11, 2013, by and on behalf of Michael Larson, filed as Exhibit 99.1 to Amendment No. 9 to Cascade's Schedule 13D with respect to Western Asset/Claymore Inflation-Linked Opportunities & Income Fund on December 11, 2013, SEC File No. 005-81261, and incorporated by reference herein. Duly authorized under Special Limited Power of Attorney appointing Alan Heuberger attorney-in-fact, dated August 12, 2008, by and on behalf of William H. Gates III, filed as Exhibit 99.2 to Amendment No. 1 to Cascade's Schedule 13D with respect to Otter Tail Corporation on April 15, 2009, SEC File No. 005-06638, and incorporated by reference herein.