X0201 4 2004-01-13 1 0000816066 APPLIED IMAGING CORP AICX 0001044321 MARXE AUSTIN W & GREENHOUSE DAVID M 153 E 53RD ST 55TH FL NEW YORK NY 10022 0 0 1 0 Common Stock 2004-01-13 4 S 0 19000 3.5285 D 1864147 I By Limited Partnerships Common Stock 2004-01-14 4 S 0 172000 2.9302 D 1692147 I By Limited Partnerships Common Stock 2004-01-14 4 S 0 12500 3.18 D 1679647 I By Limited partnerships Common Stock 2004-01-14 4 S 0 240000 2.9025 D 1439647 I By Limited Partnerships Common Stock 2004-01-15 4 S 0 66000 2.805 D 1373647 I By Limited Partnerships Common Stock 2004-01-15 4 S 0 53000 2.7055 D 1320647 I By Limited Partnerships Common Stock 2004-01-15 4 S 0 38140 2.7662 D 1282507 I By Limited Partnerships This is a joint filing by Austin W. Marxe (Marxe) and David M. Greenhouse (Greenhouse). They share voting and investment control over all securities owned by Special Situations Cayman Fund, L.P. (Cayman) and Special Situations Private Equity Fund, LP (PE), respectively. 298,575 share of Common Stock and 91,400 Wts are held by Cayman and 681,032 shares of Common Stock and 211,500 Wts are held by PE. The interest of Marxe and Greenhouse in the shares of Common Stock owned by Cayman and PE is limited to the extent of his pecuniary interest. Austin W. Marxe 2004-01-16 David M. Greenhouse 2004-01-16