<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2024-03-15</periodOfReport>

    <issuer>
        <issuerCik>0001035976</issuerCik>
        <issuerName>FNCB Bancorp, Inc.</issuerName>
        <issuerTradingSymbol>FNCB</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001067363</rptOwnerCik>
            <rptOwnerName>DENAPLES DOMINICK L</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O FNCB</rptOwnerStreet1>
            <rptOwnerStreet2>102 E. DRINKER ST.</rptOwnerStreet2>
            <rptOwnerCity>DUNMORE</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>18512</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>COMMON STOCK</value>
            </securityTitle>
            <transactionDate>
                <value>2024-03-15</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>1406.92</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>6.2156</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>98572.86</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
        <nonDerivativeHolding>
            <securityTitle>
                <value>COMMON STOCK</value>
            </securityTitle>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>2439</value>
                    <footnoteId id="F2"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>By DeNaples Equipment Company</value>
                </natureOfOwnership>
            </ownershipNature>
        </nonDerivativeHolding>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">This amount includes 130.8970 shares acquired individually by Mr. DeNaples and 1,276.023 shares acquired jointly with his spouse under the Issuer's Dividend Reinvestment and Stock Purchase Plan (&quot;DRP&quot;) in March 2024.  Therefore, Mr. DeNaples owns 9,171.0100 shares individually and 89,401.8500 shares jointly with his spouse.</footnote>
        <footnote id="F2">Comprises 2,439 shares held by a business in which Mr. DeNaples is a 33.33% owner with his brother, Louis A. DeNaples.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ James M. Bone, Jr., CPA, As Attorney in Fact</signatureName>
        <signatureDate>2024-03-19</signatureDate>
    </ownerSignature>
</ownershipDocument>
