<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2022-09-15</periodOfReport>

    <issuer>
        <issuerCik>0001035976</issuerCik>
        <issuerName>FNCB Bancorp, Inc.</issuerName>
        <issuerTradingSymbol>FNCB</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001718261</rptOwnerCik>
            <rptOwnerName>Lambert Kathleen McCarthy</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O FNCB</rptOwnerStreet1>
            <rptOwnerStreet2>102 E. DRINKER ST.</rptOwnerStreet2>
            <rptOwnerCity>DUNMORE</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>18512</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock</value>
            </securityTitle>
            <transactionDate>
                <value>2022-09-15</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>A</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>132.169</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>8.1829</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>14649.094</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">This amount includes 132.1690 shares acquired by Mrs. Lambert individually through the Issuer's Dividend Reinvestment and Stock Purchase Plan in September 2022.  Therefore, Mrs. Lambert owns 2,500 shares jointly with her spouse and 12,149.0940 shares individually.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ James M. Bone, Jr., CPA, As Attorney in Fact</signatureName>
        <signatureDate>2022-09-19</signatureDate>
    </ownerSignature>
</ownershipDocument>
