<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0306</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2021-12-15</periodOfReport>

    <issuer>
        <issuerCik>0001035976</issuerCik>
        <issuerName>FNCB Bancorp, Inc.</issuerName>
        <issuerTradingSymbol>FNCB</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001650455</rptOwnerCik>
            <rptOwnerName>Drust Richard D</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O FNCB</rptOwnerStreet1>
            <rptOwnerStreet2>102 E. DRINKER ST.</rptOwnerStreet2>
            <rptOwnerCity>DUNMORE</rptOwnerCity>
            <rptOwnerState>PA</rptOwnerState>
            <rptOwnerZipCode>18512</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isOfficer>1</isOfficer>
            <officerTitle>SVP/RETAIL BANKING OFFICER</officerTitle>
        </reportingOwnerRelationship>
    </reportingOwner>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>COMMON STOCK</value>
            </securityTitle>
            <transactionDate>
                <value>2021-12-15</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>J</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>22.5970</value>
                </transactionShares>
                <transactionPricePerShare>
                    <value>8.7693</value>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>A</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>12429.677</value>
                    <footnoteId id="F1"/>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">This amount includes 6,372 shares of time-based restricted common stock solely owned by Mr. Drust; and 6,057.6770 shares owned solely by Mr. Drust and includes 22.5970 shares acquired under the Issuer's Dividend Reinvestment and Stock Purchase Plan (&quot;DRP&quot;) for December 2021.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ James M. Bone, Jr. as Attorney in Fact</signatureName>
        <signatureDate>2021-12-17</signatureDate>
    </ownerSignature>
</ownershipDocument>
