Exhibit 24(b)(8.100)

THIRD AMENDMENT TO PARTICIPATION AGREEMENT

THIS AMENDMENT effective April 1, 2008 among ING Life Insurance and Annuity Company (ING
Life), Wells Fargo Funds Management, LLC (the Company), acting as the administrator for the registered
open-end management investment companies (each a Fund or collectively the Funds), whose shares are or may
be distributed by Wells Fargo Funds Distributor, LLC (the Distributor).

WHEREAS, ING Life, Strong Investor Services, Inc. and Strong Investments, Inc. signed a Fund
Participation Agreement effective August 19, 2002, as amended by the First Amendment to Participation
Agreement dated October 30, 2006, to reflect the assignment of the Fund Participation Agreement to Wells
Fargo Funds Management, LLC and Wells Fargo Funds Distributor, LLC, as amended by the Second
Amendment to Participation Agreement dated August 1, 2007, (together, the Agreement).

WHEREAS, ING Life, Company and Distributor desire to amend the Agreement in accordance with
the provisions of Section 11 thereof;

NOW THEREFORE, in consideration of the above premises, ING Life, Company and Distributor
hereby agree to amend the Agreement, effective as of June 20, 2008 (or as of such other date on which the
respective modifications to the Class Z, Class D and Advisor Class shares to assume the names, features and
attributes associated with Investor Class, Investor Class and Class A shares, respectively, take effect through
post-effective amendments to the registration statements of the Funds), by restating Exhibit A in its entirety, as
attached hereto. All other terms and provisions of the Agreement not amended herein shall remain in full force
and effect.

IN WITNESS WHEREOF, the parties hereto have caused this Amendment to be executed in their
names and on their behalf by and through their duly authorized officers as of the day and year first set forth
above.

ING LIFE INSURANCE AND ANNUITY		WELLS FARGO FUNDS MANAGEMENT, LLC
COMPANY
By:	/s/ Michael C. Eldredge	By:	/s/ A. Erdem Cimen
Name:	Michael C. Eldredge	Name:	A. Erdem Cimen
Title:	Vice President	Title:	SVP, Chief Financial Officer, Treasurer
		WELLS FARGO FUNDS DISTRIBUTOR, LLC
		By:	/s/ Randy Henze
		Name:	Randy Henze
		Title:	Executive Vice President

1

Schedule A

Funds and Fees to ING Life

(a) Company shall pay to ING Life a fee, calculated and paid monthly in arrears, equal to the product of: (i) the
product of (a) the number of calendar days in the applicable month divided by the number of calendar days in
that year (365 or 366, as applicable) and (b) the percentage specified below and (ii) the average daily market
value of the investment held in such Fund, or class of shares as applicable, pursuant to this Agreement
computed by totaling the aggregate investment (share net asset value multiplied by the total number of held) on
each day during the calendar month and dividing by the total number of days during such month.
Notwithstanding the foregoing, there shall be excluded from the computation of such amount the value of
shares first placed or purchased in an Account after termination of this Agreement. Fees will be paid, at
Company’s election, by wire transfer or by check. Each payment will be accompanied by a statement showing
the calculation of the fee payable to ING Life for the month (provided that such calculation shall be limited to
the following information: (i) the average daily balance by account, (ii) the annual fee rate, and (iii) the final
product of the calculation) and such other supporting data as may be reasonably requested by ING Life.

(b) The Funds subject to the Agreement and applicable annual fees are as follows:
Class/Fund Name	Annual Rate
A and Investor Shares (Equity Funds)	bps (___%)
A and Investor Shares (Fixed Income Funds)	bps (___%)
Administrator Shares (Equity Funds)	bps (___%)
Administrator Shares (Fixed Income Funds)	bps (___%)
Institutional Shares (Equity Funds)	bps (___%)
Institutional Shares (Fixed Income Funds)	bps (___%)
Equity Index Funds, Index Funds and Money Market Funds	bps (___%)
Exceptions to the rates above are as follows:
Class/Fund Name	Annual Rate
C&B Mid Cap Value A Class	bps (___%)
C&B Large Cap Value A Class	bps (___%)
C&B Mid Cap Value Admin Class	bps (___%)
C&B Large Cap Value Admin Class	bps (___%)
C&B Mid Cap Value Institutional Class	bps (___%)
C&B Large Cap Value Institutional Class	bps (___%)
C&B Mid Cap Value Investor Class	bps (___%)
C&B Large Cap Value Investor Class	bps (___%)
Small Cap Value Fund - Institutional Class	bps (___%)
Small Cap Value Fund - Investor Class	bps (___%)

(c) Fund as used in the Agreement shall be deemed to include any class of shares of any Fund.

A-1