<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0206</schemaVersion>

    <documentType>3</documentType>

    <periodOfReport>2013-04-11</periodOfReport>

    <noSecuritiesOwned>0</noSecuritiesOwned>

    <issuer>
        <issuerCik>0001313911</issuerCik>
        <issuerName>Rally Software Development Corp</issuerName>
        <issuerTradingSymbol>RALY</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001573991</rptOwnerCik>
            <rptOwnerName>Ninth MDV Partners, L.L.C.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O NINTH MDV PARTNERS, L.L.C.</rptOwnerStreet1>
            <rptOwnerStreet2>3000 SAND HILL ROAD, BLDG 3, STE 290</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001410787</rptOwnerCik>
            <rptOwnerName>MDV IX LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O NINTH MDV PARTNERS, L.L.C.</rptOwnerStreet1>
            <rptOwnerStreet2>3000 SAND HILL ROAD, BLDG 3, STE 290</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001435083</rptOwnerCik>
            <rptOwnerName>MDV ENF IX LP</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O NINTH MDV PARTNERS, L.L.C.</rptOwnerStreet1>
            <rptOwnerStreet2>3000 SAND HILL ROAD, BLDG 3, STE 290</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001503381</rptOwnerCik>
            <rptOwnerName>Ericson William W.</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O NINTH MDV PARTNERS, L.L.C.</rptOwnerStreet1>
            <rptOwnerStreet2>3000 SAND HILL ROAD, BLDG 3, STE 290</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001027621</rptOwnerCik>
            <rptOwnerName>FEIBER JONATHAN D</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>C/O NINTH MDV PARTNERS, L.L.C.</rptOwnerStreet1>
            <rptOwnerStreet2>3000 SAND HILL ROAD, BLDG 3, STE 290</rptOwnerStreet2>
            <rptOwnerCity>MENLO PARK</rptOwnerCity>
            <rptOwnerState>CA</rptOwnerState>
            <rptOwnerZipCode>94025</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>0</isDirector>
            <isOfficer>0</isOfficer>
            <isTenPercentOwner>1</isTenPercentOwner>
            <isOther>0</isOther>
        </reportingOwnerRelationship>
    </reportingOwner>

    <derivativeTable>
        <derivativeHolding>
            <securityTitle>
                <value>Series C Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F1"/>
                <footnoteId id="F2"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F1"/>
                <footnoteId id="F2"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F3"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>2650761</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>By MDV IX, L.P.</value>
                    <footnoteId id="F6"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series D Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F1"/>
                <footnoteId id="F4"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F1"/>
                <footnoteId id="F4"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F3"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>361784</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>By MDV IX, L.P.</value>
                    <footnoteId id="F6"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
        <derivativeHolding>
            <securityTitle>
                <value>Series E Preferred Stock</value>
            </securityTitle>
            <conversionOrExercisePrice>
                <footnoteId id="F1"/>
                <footnoteId id="F5"/>
            </conversionOrExercisePrice>
            <exerciseDate>
                <footnoteId id="F1"/>
                <footnoteId id="F5"/>
            </exerciseDate>
            <expirationDate>
                <footnoteId id="F3"/>
            </expirationDate>
            <underlyingSecurity>
                <underlyingSecurityTitle>
                    <value>Common Stock</value>
                </underlyingSecurityTitle>
                <underlyingSecurityShares>
                    <value>194380</value>
                </underlyingSecurityShares>
            </underlyingSecurity>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>I</value>
                </directOrIndirectOwnership>
                <natureOfOwnership>
                    <value>As nominee for MDV IX, L.P. and MDV ENF IX, L.P.</value>
                    <footnoteId id="F6"/>
                </natureOfOwnership>
            </ownershipNature>
        </derivativeHolding>
    </derivativeTable>

    <footnotes>
        <footnote id="F1">Immediately convertible into shares of the Issuer's Common Stock at the Reporting Person's election.</footnote>
        <footnote id="F2">The shares will automatically convert into 2,650,761 shares of the Issuer's Common Stock immediately upon the closing of a Qualified Public Offering (as defined in the Issuer's Amended and Restated Certificate of Incorporation, as filed with the Delaware Secretary of State on April 1, 2013).</footnote>
        <footnote id="F3">The shares do not have an expiration date.</footnote>
        <footnote id="F4">The shares will automatically convert into 361,784 shares of the Issuer's Common Stock immediately upon the closing of a Qualified Public Offering.</footnote>
        <footnote id="F5">The shares will automatically convert into 194,380 shares of the Issuer's Common Stock immediately upon the closing of a Qualified Public Offering.</footnote>
        <footnote id="F6">Includes 3,012,545 shares held by MDV IX, L.P. and 194,380 shares held by MDV IX, L.P., as nominee for MDV IX, L.P. and MDV ENF IX, L.P. (collectively, &quot;MDV IX&quot;). Ninth MDV Partners, L.L.C. (&quot;Ninth MDV&quot;) is the General Partner of MDV IX. William Ericson and Jonathan Feiber are managing members of Ninth MDV.  Each of William Ericson, Jonathan Feiber, and Ninth MDV may be deemed to share voting and dispositive power over the shares held by MDV IX.</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>Ninth MDV Partners, L.L.C. By: /s/ William W. Ericson, Managing Member</signatureName>
        <signatureDate>2013-04-11</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>MDV IX, L.P. By: Ninth MDV Partners, L.L.C., its General Partner By: /s/ Brett Teele, Authorized Signatory</signatureName>
        <signatureDate>2013-04-11</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>MDV ENF IX, L.P. By: Ninth MDV Partners, L.L.C., its General Partner By: /s/ Brett Teele, Authorized Signatory</signatureName>
        <signatureDate>2013-04-11</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ William W. Ericson</signatureName>
        <signatureDate>2013-04-11</signatureDate>
    </ownerSignature>

    <ownerSignature>
        <signatureName>/s/ Jonathan Feiber</signatureName>
        <signatureDate>2013-04-11</signatureDate>
    </ownerSignature>
</ownershipDocument>
