X0306 4 2022-01-27 0001018399 ENTERPRISE BANCORP INC /MA/ EBTC 0001637827 GALLAGHER MICHAEL JOHN C/O ENTERPRISE BANCORP, INC. 222 MERRIMACK STREET LOWELL MA 01852 1 EVP-Principal Subsidiary Common Stock 2022-01-27 4 F 0 62 41.79 D 1895.8633 D Common Stock 9470.3915 I By Trust Includes 14.5856 shares acquired through the Issuer's Dividend Reinvestment Plan on 6/1/2021. Includes 15.0971 shares acquired through the Issuer's Dividend Reinvestment Plan on 9/1/2021. Includes 7.492 shares acquired through the Issuer's Dividend Reinvestment Plan on 12/1/2021. Includes 2.0897 shares acquired through the Issuer's Employee Stock Purchase Plan on 6/4/2021. Includes 2.1388 shares acquired through the Issuer's Employee Stock Purchase Plan on 9/7/2021. Includes 1.8815 shares acquired through the Issuer's Employee Stock Purchase Plan on 12/6/2021. Report includes transfer of shares from direct ownership to indirect ownership by trust. Includes 43.2082 shares acquired through the Issuer's Dividend Reinvestment Plan on 6/1/2021. Includes 44.7243 shares acquired through the Issuer's Dividend Reinvestment Plan on 9/1/2021. Includes 39.7022 shares acquired through the Issuer's Dividend Reinvestment Plan on 12/1/2021. /s/ John P. Clancy, Jr. as attorney-in-fact for Michael John Gallagher 2022-01-31