X0202 SCHEDULE 13D/A 0001493152-25-025349 0001859356 XXXXXXXX LIVE 1 Common Stock, par value $0.01 per share 04/15/2026 false 0001014763 00902F402 Ainos, Inc.

AINOS, INC. 8880 RIO SAN DIEGO DRIVE SUITE 800 SAN DIEGO CA 92108

Chun-Hsien Tsai 886-37-581999 14F., No. 61, Sec. 4, New Taipei Boulevard, Xinzhuang District New Taipei City F5 242 0001859356 N Chun-Jung Tsai OO N F5 0.00 686999.00 686999.00 0.00 686999.00 N 8.06 OO The numbers in Rows 8, 9, and 11 represents beneficial ownership of 686,999 shares of common stock, $0.01 par value (the "Common Stock"), of Ainos, Inc., a Texas corporation (the "Issuer"), which are subject to a voting agreement between the Reporting Person and Ainos Inc., a Cayman Islands corporation ("Ainos KY"). For further details, please see Item 3 and Item 6. The percentage in Row 13 is based on the sum of (i) 7,266,011 shares of Common Stock outstanding as of March 30, 2026 as set forth in the Annual Report on Form 10-K of the Issuer filed with the SEC on March 30, 2026, (ii) 19,531 shares issued for service fee on April 1, 2026; and (iii) 1,239,000 shares of Common Stock granted and vested on April 15, 2026 under the Ainos, Inc. 2023 Stock Incentive Plan. Common Stock, par value $0.01 per share Ainos, Inc. AINOS, INC. 8880 RIO SAN DIEGO DRIVE SUITE 800 SAN DIEGO CA 92108 This Amendment No. 1 ("Amendment No. 1") amends the Schedule 13D first filed with the Securities and Exchange Commission on November 28, 2025 (the "Schedule 13D"), and is filed by Chun-Jung Tsai, with respect to the common stock, par value $0.001 per share (the "Common Stock"), of Ainos, Inc. (the "Issuer"). Unless otherwise indicated, all capitalized terms used and not defined herein have the respective meanings assigned to them in the Schedule 13D. The Reporting Person acquired 330,000 restricted stock units ("RSUs") of Common Stock to the Reporting Person under the Ainos, Inc. 2023 Stock Incentive Plan, all of which are fully vested. The Reporting Person acquired the 330,000 RSUs under the Ainos, Inc. 2023 Stock Incentive Plan. Except as described in this Schedule 13D, the Reporting Person do not have any present plans or proposals that relate to or would result in any of the actions described in subparagraphs (a) through (j) of Item 4 of Schedule 13D, although, subject to the agreements described herein, the Reporting Person, at any time, and from time to time, may review, reconsider and change their position and/or change their purpose and/or develop such plans and may seek to influence management of the Issuer or the Board of Directors with respect to the business and affairs of the Issuer and may from time to time consider pursuing or proposing such matters with advisors, the Issuer, or other persons. The information requested is incorporated herein by reference to the cover pages to this Statement. The information requested is incorporated herein by reference to the cover pages to this Statement. The reporting person has not effected any transactions in Common Stock during the past 60 days, except as described in this Statement. The information in Item 2 is incorporated by reference into this Item 5(d). Not applicable. Chun-Jung Tsai /s/ Chun-Jung Tsai 04/17/2026