X0202 SCHEDULE 13D/A 0001493152-26-004494 0001859309 XXXXXXXX LIVE 15 Common Stock, par value $0.01 per share 04/15/2026 false 0001014763 00902F402 Ainos, Inc.

3050 Post Oak Blvd Suite 510-T80 Houston TX 77056

Chun-Hsien Tsai 886-37-581999 14F.,No. 61, Sec. 4, New Taipei Bouleva, Xinzhuang District New Taipei City 242 F5 F5 0001859309 N AINOS INC a OO N E9 4807506.00 0.00 491263.00 0.00 491263.00 N 5.76 CO The number of securities in Row 7 includes (i) 491,263 shares of common stock, $0.01 par value, of Ainos, Inc., a Texas corporation (the "Issuer"), owned directly by Ainos Inc., a Cayman Islands company ("Ainos KY"), (ii) 3,649,158 shares pursuant to a Voting Agreement dated January 1, 2026 (the "2026 Voting Agreement"), by and among the Reporting Person, and Chun-Hsien Tsai, Ting Chuan Lee, Chun-Jung Tsai, Chung-Yi Tsai, Chih-Heng Lu, Taiwan Carbon Nano Technology Corporation ("TCNT"), and Hsin-Liang Lee, and (iii) 667,085 shares pursuant to a Voting Agreement dated May 3, 2024 between Ainos KY and ASE Test, Inc. ("ASE Test" and the "ASE Voting Agreement," together with the 2026 Voting Agreement, the "Voting Agreements") (with the 667,085 ASE Test shares consisting of the following (a) 11,777 shares owned by ASE Test, (b) 105,868 shares issuable to ASE Test upon conversion of outstanding convertible notes of the Issuer (c) 449,440 shares issuable to ASE Test upon conversion of a convertible note of the Issuer issuable within 60 days), and (d) 100,000 shares issuable to ASE Test upon exercise of warrants to purchase 100,000 shares of the Issuer with ASE Test). The numbers in Rows 9, 11 and 13 represents beneficial ownership of 491,263 shares of common stock of the Issuer, consisting of 491,263 shares owned directly by Ainos KY and excludes 4,316,243 shares pursuant to the Voting Agreements. The percentage in Row 13 is based on the sum of, (i) 7,266,011 shares of Common Stock outstanding as of March 30, 2026 as set forth in the Annual Report on Form 10-K of the Issuer filed with the SEC on March 30, 2026, (ii) 19,531 shares issued for service fee on April 1, 2026; and (iii) 1,239,000 shares of Common Stock granted and vested on April 15, 2026 under the Ainos, Inc. 2023 Stock Incentive Plan. Common Stock, par value $0.01 per share Ainos, Inc. 3050 Post Oak Blvd Suite 510-T80 Houston TX 77056 This Amendment No. 15 ("Amendment No. 15") amends the Schedule 13D first filed with the Securities and Exchange Commission on April 28, 2021 (the "Original Statement"), as amended and restated by Amendment No. 1 dated December 13, 2023, as amended by Amendment No. 2 dated March 8, 2022, Amendment No. 3 dated September 2, 2022, Amendment No. 4 dated August 15, 2023, Amendment No. 5 dated January 29, 2024, Amendment No. 6 dated March 11, 2024, Amendment No. 7 dated May 6, 2024, Amendment No.8 dated August 20, 2024, Amendment No.9 dated November 26, 2024, Amendment No.10 dated March 12, 2025, Amendment No. 11 dated April 9, 2025, Amendment No. 12 dated October 9, 2025, Amendment No. 13 dated November 28, 2025, and Amendment No. 14 dated January 30, 2026 (the Original Statement, as so amended shall be known as the "Statement"), with respect to the common stock, par value $0.001 per share (the "Common Stock"), of Ainos, Inc. (the "Issuer"). Unless otherwise indicated, all capitalized terms used and not defined herein have the respective meanings assigned to them in the Schedule 13D. Since the filing of Amendment No. 14 on January 30, 2026, Mr. Chun-Hsien Tsai, Mrs. Ting-Chuan Lee and Mr. Chun-Jung Tsai were granted restricted stock units ("RSUs") of the Issuer on April 15, 2026, in the amounts of 300,000, 570,000 and 330,000, respectively, under the Ainos, Inc. 2023 Stock Incentive Plan. The RSUs fully vested on the same day. Pursuant to the 2026 Voting Agreement, the reporting person has sole voting discretion over such shares. The information requested by this Item 3 is incorporated herein by reference to Item 5 hereof. Ainos KY acquired the Common stock in the Issuer for investment purposes. Except as disclosed herein, each of the parties has no plans which relate to or would result in an event described in subparagraphs (a) through (j) of Item 4 of Schedule 13D. However, each of the Parties may, from time to time, engage in discussions, whether initiated by the Parties or another party, concerning proposals for transactions or other arrangements that may relate to or, if consummated, result in an event described in Item 4 of Schedule 13D. Each of the Parties may review and evaluate their respective investments in the Issuer at any time, whether in light of the discussions described in the immediately preceding sentence or otherwise, which may give rise to plans or proposals that, if consummated, would result in one or more of the events described in Item 4 of Schedule 13D. Any such discussion or actions may consider various factors, including, without limitation, the Issuer's business prospects and other developments concerning the Issuer, alternative investment opportunities, general economic conditions, financial and stock market conditions and any other facts and circumstances that may become known to the Parties regarding or related to the matters described in this Statement. Items 5 of the Statement are hereby amended and restated as follows: (a) - (b) The information requested by these paragraphs are incorporated herein by reference to the cover pages to this Amendment No. 15. The reporting person has not effected any transactions in Common Stock during the past 60 days, except as described in this Statement. No other person is known to the reporting person to have the right to receive or the power to direct the receipt of dividends or the proceeds from the sale of the shares (other than their respective records owner). Not applicable. None. AINOS INC /s/ Chun-Hsien Tsai 04/17/2026