<?xml version="1.0"?>
<ownershipDocument>

    <schemaVersion>X0508</schemaVersion>

    <documentType>4</documentType>

    <periodOfReport>2025-08-12</periodOfReport>

    <notSubjectToSection16>1</notSubjectToSection16>

    <issuer>
        <issuerCik>0001008586</issuerCik>
        <issuerName>STREAMLINE HEALTH SOLUTIONS INC.</issuerName>
        <issuerTradingSymbol>STRM</issuerTradingSymbol>
    </issuer>

    <reportingOwner>
        <reportingOwnerId>
            <rptOwnerCik>0001400173</rptOwnerCik>
            <rptOwnerName>Starkey Judith</rptOwnerName>
        </reportingOwnerId>
        <reportingOwnerAddress>
            <rptOwnerStreet1>2400 OLD MILTON PARKWAY</rptOwnerStreet1>
            <rptOwnerStreet2>BOX 1353</rptOwnerStreet2>
            <rptOwnerCity>ALPHARETTA</rptOwnerCity>
            <rptOwnerState>GA</rptOwnerState>
            <rptOwnerZipCode>30009</rptOwnerZipCode>
            <rptOwnerStateDescription></rptOwnerStateDescription>
        </reportingOwnerAddress>
        <reportingOwnerRelationship>
            <isDirector>1</isDirector>
        </reportingOwnerRelationship>
    </reportingOwner>

    <aff10b5One>0</aff10b5One>

    <nonDerivativeTable>
        <nonDerivativeTransaction>
            <securityTitle>
                <value>Common Stock, $0.01 par value</value>
            </securityTitle>
            <transactionDate>
                <value>2025-08-12</value>
            </transactionDate>
            <transactionCoding>
                <transactionFormType>4</transactionFormType>
                <transactionCode>D</transactionCode>
                <equitySwapInvolved>0</equitySwapInvolved>
                <footnoteId id="F1"/>
            </transactionCoding>
            <transactionAmounts>
                <transactionShares>
                    <value>75820</value>
                </transactionShares>
                <transactionPricePerShare>
                    <footnoteId id="F1"/>
                </transactionPricePerShare>
                <transactionAcquiredDisposedCode>
                    <value>D</value>
                </transactionAcquiredDisposedCode>
            </transactionAmounts>
            <postTransactionAmounts>
                <sharesOwnedFollowingTransaction>
                    <value>0</value>
                </sharesOwnedFollowingTransaction>
            </postTransactionAmounts>
            <ownershipNature>
                <directOrIndirectOwnership>
                    <value>D</value>
                </directOrIndirectOwnership>
            </ownershipNature>
        </nonDerivativeTransaction>
    </nonDerivativeTable>

    <footnotes>
        <footnote id="F1">This Form 4 reports securities disposed pursuant to the Agreement and Plan of Merger (the &quot;Merger Agreement&quot;), dated as of May 29, 2025, by and among the Issuer, Mist Holding Co. (&quot;Parent&quot;), and MD BE Merger Sub, Inc., a wholly owned subsidiary of Parent (&quot;Merger Sub&quot;), pursuant to which Merger Sub merged with and into the Issuer, effective as of August 12, 2025, with the Issuer surviving the Merger as a wholly owned subsidiary of Parent (the &quot;Merger&quot;). Pursuant to the terms of the Merger Agreement, at the effective time of the Merger (the &quot;Effective Time&quot;), each share of common stock, par value $0.01 per share, of the Company (&quot;Common Stock&quot;) issued and outstanding as of immediately prior to the Effective Time was canceled and converted into the right to receive $5.34 in cash, without interest (the &quot;Merger Consideration&quot;).</footnote>
    </footnotes>

    <ownerSignature>
        <signatureName>/s/ Judith Starkey</signatureName>
        <signatureDate>2025-08-14</signatureDate>
    </ownerSignature>
</ownershipDocument>
