Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

As independent registered public accountants, we hereby consent to the use of our report dated October 26, 2017, relating to the consolidated financial statements of PURE Bioscience, Inc., appearing in this Post-Effective Amendment No. 2 to Form S-1 (No. 333-215915), and to the reference to us under the heading “Experts”.

/s/ Mayer Hoffman McCann P.C.

San Diego, California

October 27, 2017