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      <liveTestFlag>LIVE</liveTestFlag>
      <filer>
        <issuerCredentials>
          <cik>0001005336</cik>
          <ccc>XXXXXXXX</ccc>
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      <flags>
        <overrideInternetFlag>false</overrideInternetFlag>
        <confirmingCopyFlag>false</confirmingCopyFlag>
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      <investmentCompanyType>N-4</investmentCompanyType>
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      <reportClass>
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    <generalInfo reportEndingPeriod="2025-12-31" isReportPeriodLt12="N"/>
    <registrantInfo>
      <registrantFullName>Jefferson National Life Annuity Account G</registrantFullName>
      <investmentCompFileNo>811-07501</investmentCompFileNo>
      <registrantCik>0001005336</registrantCik>
      <registrantLei>254900BOJ6RKD1GZFF92</registrantLei>
      <registrantstreet1>One Nationwide Plaza</registrantstreet1>
      <registrantcity>Columbus</registrantcity>
      <registrantzipCode>43215-2226</registrantzipCode>
      <registrantstate>US-OH</registrantstate>
      <registrantcountry>US</registrantcountry>
      <registrantphoneNumber>614-249-7111</registrantphoneNumber>
      <websites>
        <website webpage="www.nationwideadvisory.com"/>
      </websites>
      <locationBooksRecords>
        <locationBooksRecord>
          <officeName>Jefferson National Life Insurance Company</officeName>
          <officeAddress1>One Nationwide Plaza</officeAddress1>
          <officeCity>Columbus</officeCity>
          <officeStateCountry officeState="US-OH" officeCountry="US"/>
          <officeRecordsZipCode>43215-2226</officeRecordsZipCode>
          <officePhone>614-249-7111</officePhone>
          <booksRecordsDesc>The accounts, books, and other documents relating to the business of Jefferson National Life Annuity Account G which constitute the record forming the basis for financial statements required to be filed pursuant to section 30 of the Investment Company Act of 1940 and of the auditor's certificates relating thereto. </booksRecordsDesc>
        </locationBooksRecord>
      </locationBooksRecords>
      <isRegistrantFirstFiling>N</isRegistrantFirstFiling>
      <isRegistrantLastFiling>N</isRegistrantLastFiling>
      <registrantFamilyInvComp isRegistrantFamilyInvComp="Y" familyInvCompFullName="Nationwide Registered Separate Accounts"/>
      <registrantClassificationType>N-4</registrantClassificationType>
      <isSecuritiesActRegistration>Y</isSecuritiesActRegistration>
      <chiefComplianceOfficers>
        <chiefComplianceOfficer>
          <ccoName>Kevin Douglas Grether</ccoName>
          <crdNumber>002261829</crdNumber>
          <ccoStreet1>One Nationwide Plaza</ccoStreet1>
          <ccoCity>Columbus</ccoCity>
          <ccoStateCountry ccoState="US-OH" ccoCountry="US"/>
          <ccoZipCode>43215-2226</ccoZipCode>
          <ccoPhone>XXXXXX</ccoPhone>
          <isCcoChangedSinceLastFiling>Y</isCcoChangedSinceLastFiling>
          <ccoEmployers>
            <ccoEmployer ccoEmployerName="N/A" ccoEmployerId="N/A"/>
          </ccoEmployers>
        </chiefComplianceOfficer>
      </chiefComplianceOfficers>
      <isRegistrantSubmittedMatter>N</isRegistrantSubmittedMatter>
      <isPreviousLegalProceeding>N</isPreviousLegalProceeding>
      <isPreviousProceedingTerminated>N</isPreviousProceedingTerminated>
      <isFinancialSupportDuringPeriod>N</isFinancialSupportDuringPeriod>
      <isExemptionFromAct>N</isExemptionFromAct>
      <principalUnderwriters>
        <principalUnderwriter>
          <principalUnderwriterName>Nationwide Investment Services</principalUnderwriterName>
          <principalUnderwriterFileNumber>8-20254</principalUnderwriterFileNumber>
          <principalUnderwriterCrdNumber>000007110</principalUnderwriterCrdNumber>
          <principalUnderwriterLei>N/A</principalUnderwriterLei>
          <principalUnderwriterRssdId>N/A</principalUnderwriterRssdId>
          <principalUnderWriterStateCountry principalUnderWriterState="US-OH" principalUnderWriterCountry="US"/>
          <isPrincipalUnderwriterAffiliatedWithRegistrant>Y</isPrincipalUnderwriterAffiliatedWithRegistrant>
        </principalUnderwriter>
      </principalUnderwriters>
      <isUnderwriterHiredOrTerminated>N</isUnderwriterHiredOrTerminated>
      <publicAccountants>
        <publicAccountant>
          <publicAccountantName>KPMG, LLP</publicAccountantName>
          <pcaobNumber>185</pcaobNumber>
          <publicAccountantLei>5493003PVCIGA21K0K42</publicAccountantLei>
          <publicAccountantRssdId>N/A</publicAccountantRssdId>
          <publicAccountantStateCountry publicAccountantState="US-DE" publicAccountantCountry="US"/>
        </publicAccountant>
      </publicAccountants>
      <isPublicAccountantChanged>N</isPublicAccountantChanged>
      <isOpinionOffered>N</isOpinionOffered>
      <isMaterialChange>N</isMaterialChange>
      <isAccountingPrincipleChange>N</isAccountingPrincipleChange>
    </registrantInfo>
    <unitInvestmentTrust>
      <depositors>
        <depositor>
          <depositorName>Jefferson National Life Insurance Company</depositorName>
          <depositorCrdNo>N/A</depositorCrdNo>
          <depositorLei>N/A</depositorLei>
          <depositorRssdId>N/A</depositorRssdId>
          <depositorStateCountry depositorState="US-KY" depositorCountry="US"/>
          <depositorUltimateParentname>Nationwide Mutual Insurance Company</depositorUltimateParentname>
        </depositor>
      </depositors>
      <uitAdmins>
        <uitAdmin>
          <uitAdminName>Jefferson National Life Insurance Company</uitAdminName>
          <uitAdminLei>N/A</uitAdminLei>
          <uitAdminRssdId>N/A</uitAdminRssdId>
          <uitAdminStateCountry uitAdminState="US-TX" uitAdminCountry="US"/>
          <isUitAdminAffiliated>Y</isUitAdminAffiliated>
          <isUitAdminSubAdmin>N</isUitAdminSubAdmin>
        </uitAdmin>
      </uitAdmins>
      <isUitAdminHiredTerminated>N</isUitAdminHiredTerminated>
      <registrantSeparateInsuranceAccount isRegistrantSeparateInsuranceAccount="Y" separateAccountSeriesId="S000005256"/>
      <numOfContracts>33099</numOfContracts>
      <contractSecurities>
        <contractSecurity separateAccountSecurityName="Monument Contract No. 22-4056" separateAccountContractId="C000014319" separateAccountTotalAsset="13895999192.370000000000" numContractsSold="3809" grossPremiumReceived="1572978567.850000000000" grossPremiumReceivedSection1035="1433282765.750000000000" numContractsAffected="22131" contractValueRedeemed="725713803.870000000000" contractValueRedeemedSection1035="606918011.250000000000" numContractsAffectedRedeemed="3567"/>
        <contractSecurity separateAccountSecurityName="Monument Advisor Contract No. JNL-2300" separateAccountContractId="C000014324" separateAccountTotalAsset="0.000000000000" numContractsSold="0" grossPremiumReceived="0.000000000000" grossPremiumReceivedSection1035="0.000000000000" numContractsAffected="0" contractValueRedeemed="0.000000000000" contractValueRedeemedSection1035="0.000000000000" numContractsAffectedRedeemed="0"/>
        <contractSecurity separateAccountSecurityName="Monument Advisor Select" separateAccountContractId="C000220422" separateAccountTotalAsset="0.000000000000" numContractsSold="0" grossPremiumReceived="0.000000000000" grossPremiumReceivedSection1035="0.000000000000" numContractsAffected="0" contractValueRedeemed="0.000000000000" contractValueRedeemedSection1035="0.000000000000" numContractsAffectedRedeemed="0"/>
      </contractSecurities>
      <isRule6C7Reliance>N</isRule6C7Reliance>
      <isRule11A2Reliance>N</isRule11A2Reliance>
      <isRule12D1Dash4Reliance>N</isRule12D1Dash4Reliance>
      <isRule12D1GReliance>N</isRule12D1GReliance>
    </unitInvestmentTrust>
    <signature registrantSignedName="JEFFERSON NATIONAL LIFE ANNUITY ACCOUNT G" signedDate="2026-03-11" signature="Nic Staley" title="AVP, Financial Reporting"/>
  </formData>
</edgarSubmission>
