Exhibit 23.1

Assentsure PAC UEN — 201816648N 180B Bencoolen Street 03-01 The Bencoolen Singapore 189648
http://www.assentsure.com.sg

Consent of Independent Registered Public Accounting Firm

We consent to the incorporation by reference in this Registration Statement on Form S-8 of our report dated April 30, 2024, relating to the consolidated financial statements of Xiao-I Corporation, appearing in its Annual Report on Form 20-K for the year ended December 31, 2023. Our report includes an explanatory paragraph regarding the Company’s ability to continue as a going concern.

/s/ Assentsure PAC

Assentsure PAC
Singapore

April 10, 2025