<?xml version="1.0" encoding="US-ASCII" ?>
    <!-- Field: Doc-Info; Name: Generator; Value: GoFiler Complete; Version: 3.3e -->
    <!-- Field: Doc-Info; Name: VendorURI; Value: http://www.novaworks.co -->
    <!-- Field: Doc-Info; Name: Source; Value: EatonVance497_29964.xfr; Date: 2012/11/26T19:18:54 -->
    <!-- Field: Doc-Info; Name: Status; Value: 0x00000000 -->
<xbrli:xbrl xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xmlns:xbrldi="http://xbrl.org/2006/xbrldi" xmlns:dei="http://xbrl.sec.gov/dei/2012-01-31" xmlns:iso4217="http://www.xbrl.org/2003/iso4217" xmlns:us-types="http://fasb.org/us-types/2012-01-31" xmlns:rr="http://xbrl.sec.gov/rr/2012-01-31" xmlns:EVMFT="http://eatonvance.com/20121113">
    <link:schemaRef xlink:href="evmft-20121113.xsd" xlink:type="simple" />
    <xbrli:context id="AsOf2012-11-13">
      <xbrli:entity>
        <xbrli:identifier scheme="http://www.sec.gov/CIK">0000745463</xbrli:identifier>
      </xbrli:entity>
      <xbrli:period>
        <xbrli:startDate>2012-11-13</xbrli:startDate>
        <xbrli:endDate>2012-11-13</xbrli:endDate>
      </xbrli:period>
    </xbrli:context>
    <xbrli:context id="AsOf2012-11-13_S000005290Member">
      <xbrli:entity>
        <xbrli:identifier scheme="http://www.sec.gov/CIK">0000745463</xbrli:identifier>
        <xbrli:segment>
          <xbrldi:explicitMember dimension="dei:LegalEntityAxis">EVMFT:S000005290Member</xbrldi:explicitMember>
        </xbrli:segment>
      </xbrli:entity>
      <xbrli:period>
        <xbrli:startDate>2012-11-13</xbrli:startDate>
        <xbrli:endDate>2012-11-13</xbrli:endDate>
      </xbrli:period>
    </xbrli:context>
    <xbrli:unit id="USD">
      <xbrli:measure>iso4217:USD</xbrli:measure>
    </xbrli:unit>
    <xbrli:unit id="Ratio">
      <xbrli:measure>xbrli:pure</xbrli:measure>
    </xbrli:unit>
    <dei:DocumentType contextRef="AsOf2012-11-13">Other</dei:DocumentType>
    <dei:DocumentPeriodEndDate contextRef="AsOf2012-11-13">2012-10-31</dei:DocumentPeriodEndDate>
    <dei:EntityRegistrantName contextRef="AsOf2012-11-13">Eaton Vance Mutual Funds Trust</dei:EntityRegistrantName>
    <dei:EntityCentralIndexKey contextRef="AsOf2012-11-13">0000745463</dei:EntityCentralIndexKey>
    <dei:AmendmentFlag contextRef="AsOf2012-11-13">false</dei:AmendmentFlag>
    <dei:DocumentCreationDate contextRef="AsOf2012-11-13">2012-11-13</dei:DocumentCreationDate>
    <dei:DocumentEffectiveDate contextRef="AsOf2012-11-13">2012-11-13</dei:DocumentEffectiveDate>
    <rr:ProspectusDate contextRef="AsOf2012-11-13">2012-03-01</rr:ProspectusDate>
    <EVMFT:SupplementTextBlock contextRef="AsOf2012-11-13_S000005290Member">&lt;p style="margin: 0"&gt;&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 18.7pt; text-align: center"&gt;EATON VANCE LOW DURATION FUND&lt;br /&gt;&#13;&lt;br /&gt;&#13;Supplement to Prospectus dated March 1, 2012&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 5.6pt"&gt;1. &amp;#160;Effective November 13, 2012 the name of the Fund&#13;has been changed to &amp;#147;Eaton Vance Low Duration Government Income Fund&amp;#148;.&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 5.6pt"&gt;2. &amp;#160;The following replaces the first two paragraphs&#13;and bullet points under &amp;#147;Principal Investment Strategies&amp;#148; in &amp;#147;Fund Summaries &amp;#150; Eaton Vance Low Duration&#13;Fund&amp;#148;:&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 5.6pt"&gt;Under normal circumstances, the Fund invests at least 80%&#13;of the Fund&amp;#146;s net assets in securities issued, backed or otherwise guaranteed by the U.S. Government, its agencies or instrumentalities&#13;(the &amp;#147;80% Policy&amp;#148;). The Fund may invest up to 10% of its net assets in securities in any ratings category. &amp;#160;The&#13;Fund&amp;#146;s dollar-weighted average duration will not exceed three years.&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 5.6pt"&gt;The Fund seeks to achieve its investment objective by primarily&#13;allocating assets among other registered investment companies sponsored by the Eaton Vance organization that invest in various&#13;asset classes (the &amp;#147;Portfolios&amp;#148;) (commonly known as a &amp;#147;fund-of-funds&amp;#148;). &amp;#160;The Fund&amp;#146;s portfolio&#13;manager, taking market and other factors into consideration, determines the percentage of the Fund&amp;#146;s assets invested in each&#13;Portfolio. &amp;#160;Each Portfolio is described in &amp;#147;Further Information about the Portfolios&amp;#148; in this Prospectus.&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 5.6pt"&gt;3. &amp;#160;The following is added following the second paragraph&#13;under &amp;#147;Investment Objectives &amp;#38; Principal Policies and Risks&amp;#148;:&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 5.6pt"&gt;Low Duration Government Income Fund currently seeks its&#13;objective by primarily investing in one or more of the following Portfolios:&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 22.45pt"&gt;&lt;font style="font-family: Symbol"&gt;&amp;#183;&amp;#9;&lt;/font&gt;CMBS&#13;Portfolio;&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 22.45pt"&gt;&lt;font style="font-family: Symbol"&gt;&amp;#183;&amp;#9;&lt;/font&gt;Floating&#13;Rate Portfolio;&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 22.45pt"&gt;&lt;font style="font-family: Symbol"&gt;&amp;#183;&amp;#9;&lt;/font&gt;Government&#13;Obligations Portfolio;&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 22.45pt"&gt;&lt;font style="font-family: Symbol"&gt;&amp;#183;&amp;#9;&lt;/font&gt;Short&#13;Duration High Income Portfolio; and&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0; text-indent: 22.45pt"&gt;&lt;font style="font-family: Symbol"&gt;&amp;#183;&amp;#9;&lt;/font&gt;Short-Term&#13;U.S. Government Portfolio&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0"&gt;&amp;#160;&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 5.6pt"&gt;4. &amp;#160;The following replaces the paragraph &amp;#147;Short-Term&#13;U.S. Government Portfolio (formerly &amp;#147;Investment Portfolio&amp;#148;).&amp;#148; under &amp;#147;Further Information about the Portfolios&amp;#148;:&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0 0 5.6pt"&gt;&lt;font style="color: #3F3F3F"&gt;Short-Term U.S. Government&#13;Portfolio (formerly &amp;#147;Investment Portfolio&amp;#148;). &amp;#160;&lt;/font&gt;&lt;font style="color: black"&gt;The Portfolio&amp;#146;s investment&#13;objective is to seek total return. &amp;#160;Under normal circumstances, the Portfolio will invest at least 80% of its net assets in&#13;securities issued, backed or otherwise guaranteed by the U.S. Government or its agencies or instrumentalities (the &amp;#147;80% Policy&amp;#148;).&#13;&amp;#160;The Portfolio may also invest in mortgage-backed securities, corporate bonds, preferred stocks, asset-backed securities and/or&#13;money market securities. &amp;#160;Additionally, the Portfolio&amp;#146;s dollar-weighted average duration will not exceed three years.&#13;&amp;#160;The Portfolio may engage in active management techniques, securities lending and borrowing. &amp;#160;The Portfolio may borrow&#13;from banks to increase investments (&amp;#147;leveraging&amp;#148;). &amp;#160;Such borrowings will be unsecured. &amp;#160;The Portfolio may&#13;borrow an amount (when taken together with any borrowings for temporary purposes) equal to as much as 50% of the value of its net&#13;assets (not including such borrowings). &amp;#160;Leveraging will exaggerate any increase or decrease in the net asset value of the&#13;securities held by the Portfolio and, in that respect, may be considered a speculative practice. &amp;#160;In addition, the costs associated&#13;with borrowing may exceed the return on investments acquired with borrowed funds. &amp;#160;The Portfolio is authorized to participate&#13;in non-recourse lending programs. &amp;#160;The Portfolio intends to limit its cumulative net investment in such program assets to&#13;5% of its total net assets determined at the time of investment. The Portfolio may enter into forward commitments to purchase Generic&#13;MBS, with the total amount of such outstanding commitments not to exceed 10% of the Portfolio&amp;#146;s total net assets. &amp;#160;Such&#13;forward commitments may be entered into for purposes of investment leverage. &amp;#160;The Portfolio may enter into forward commitments&#13;to sell Generic MBS, with the total amount of such outstanding commitments not to exceed 50% of the Portfolio&amp;#146;s MBS holdings.&#13;&amp;#160;In addition, the Portfolio at times may enter into mortgage dollar rolls. &amp;#160;The portfolio may engage in short sales of&#13;securities. &amp;#160;The Portfolio may also take short or long positions with regard to certain Markit indices which are synthetic&#13;total return swap indices. &amp;#160;The Portfolio may engage in securities lending for total return as well as for income, and may&#13;invest the collateral received from loans in securities in which it may invest.&lt;/font&gt;&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0"&gt;&amp;#160;&lt;/p&gt;&#13;&#13;&lt;p style="font: 10pt Times New Roman, Times, Serif; margin: 0"&gt;&amp;#160;November 13, 2012&amp;#9;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;&amp;#160;&amp;#160;&amp;#160;&#13;6430-11/12 &amp;#160;&amp;#160;&amp;#160;DGHLPS2&lt;/p&gt;</EVMFT:SupplementTextBlock>
</xbrli:xbrl>
