Exhibit 21.1

Freeline Therapeutics Holdings plc*

List of Subsidiaries

Subsidiary	Jurisdiction
Freeline Holdings (UK) Limited	England and Wales
Freeline Therapeutics Limited	England and Wales
Freeline Therapeutics GmbH	Germany
Freeline Therapeutics (Ireland) Limited	Ireland
Freeline Therapeutics, Inc.	Delaware

*Following the completion of the corporate reorganization described in the prospectus that forms a part of the registration statement to which this list of subsidiaries is an exhibit.