Exhibit 23.1

CONSENT OF INDEPENDENT REGISTERED PUBLIC ACCOUNTING FIRM

We hereby consent to the incorporation by reference in this Registration Statement on Form S-3 of F-star Therapeutics, Inc. of our report dated March 30, 2021 relating to the financial statements of F-star Therapeutics, Inc., which appears in F-star Therapeutics, Inc. ‘s Annual Report on Form 10-K for the year ended December 31, 2020. We also consent to the reference to us under the heading “Experts” in such Registration Statement.

/s/PricewaterhouseCoopers LLP

Cambridge, United Kingdom

August 13, 2021

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T: +44 (0) 1223 460 055, F: +44 (0) 1223 552 336, www.pwc.co.uk

PricewaterhouseCoopers LLP is a limited liability partnership registered in England with registered number OC303525. The registered office of

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