<?xml version="1.0" encoding="us-ascii"?>
<!--XBRL document created by RR Donnelley IM Processor 5.0.0.0 using Fujitsu Interstage XWand-->
<xbrl xmlns:trpmsapi3="http://troweprice.com/20121115" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:dei="http://xbrl.sec.gov/dei/2012-01-31" xmlns:rr="http://xbrl.sec.gov/rr/2012-01-31" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:xbrldi="http://xbrl.org/2006/xbrldi" xmlns:iso4217="http://www.xbrl.org/2003/iso4217" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns="http://www.xbrl.org/2003/instance">
  <link:schemaRef xlink:type="simple" xlink:href="trpmsapi3-20121115.xsd" />
  <context id="Duration_02Jul2011_01Jul2012">
    <entity>
      <identifier scheme="http://www.sec.gov/CIK">0001533490</identifier>
    </entity>
    <period>
      <startDate>2011-07-02</startDate>
      <endDate>2012-07-01</endDate>
    </period>
  </context>
  <context id="Duration_02Jul2011_01Jul2012S000036338_Member">
    <entity>
      <identifier scheme="http://www.sec.gov/CIK">0001533490</identifier>
      <segment>
        <xbrldi:explicitMember dimension="dei:LegalEntityAxis">trpmsapi3:S000036338Member</xbrldi:explicitMember>
      </segment>
    </entity>
    <period>
      <startDate>2011-07-02</startDate>
      <endDate>2012-07-01</endDate>
    </period>
  </context>
  <context id="Duration_02Jul2011_01Jul2012S000036338_MemberC000111137_Member">
    <entity>
      <identifier scheme="http://www.sec.gov/CIK">0001533490</identifier>
      <segment>
        <xbrldi:explicitMember dimension="dei:LegalEntityAxis">trpmsapi3:S000036338Member</xbrldi:explicitMember>
        <xbrldi:explicitMember dimension="rr:ProspectusShareClassAxis">trpmsapi3:C000111137Member</xbrldi:explicitMember>
      </segment>
    </entity>
    <period>
      <startDate>2011-07-02</startDate>
      <endDate>2012-07-01</endDate>
    </period>
  </context>
  <unit id="pure">
    <measure>pure</measure>
  </unit>
  <unit id="USD">
    <measure>iso4217:USD</measure>
  </unit>
  <dei:EntityRegistrantName contextRef="Duration_02Jul2011_01Jul2012">T. Rowe Price Multi-Sector Account Portfolios, Inc.</dei:EntityRegistrantName>
  <dei:EntityCentralIndexKey contextRef="Duration_02Jul2011_01Jul2012">0001533490</dei:EntityCentralIndexKey>
  <dei:DocumentType contextRef="Duration_02Jul2011_01Jul2012">Other</dei:DocumentType>
  <dei:AmendmentFlag contextRef="Duration_02Jul2011_01Jul2012">false</dei:AmendmentFlag>
  <dei:DocumentCreationDate contextRef="Duration_02Jul2011_01Jul2012">2012-11-15</dei:DocumentCreationDate>
  <dei:DocumentEffectiveDate contextRef="Duration_02Jul2011_01Jul2012">2012-11-15</dei:DocumentEffectiveDate>
  <dei:DocumentPeriodEndDate contextRef="Duration_02Jul2011_01Jul2012">2012-11-15</dei:DocumentPeriodEndDate>
  <rr:RiskReturnHeading contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="MARGIN-TOP: 0px; MARGIN-BOTTOM: 0px" align="left"&gt;&lt;br/&gt;&lt;font style="font-family: sans-serif; font-size: 15pt; "&gt;T. Rowe Price&lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="text-align: left; font-family: sans-serif; color: rgb(0, 79, 124); margin-left: 0pt; font-size: 24pt; "&gt;Floating Rate Multi-Sector Account Portfolio&lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="FONT-FAMILY: Sans-Serif; COLOR: #004f7c; FONT-SIZE: 12pt; FONT-WEIGHT: bold"&gt;SUMMARY&lt;/font&gt;&lt;/font&gt;</rr:RiskReturnHeading>
  <rr:ObjectiveHeading contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: sans-serif; color: rgb(0, 79, 124); font-size: 9.5pt; "&gt;&lt;b&gt;Investment Objective&lt;/b&gt;&lt;/font&gt;</rr:ObjectiveHeading>
  <rr:ObjectivePrimaryTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;The fund seeks high current income and,&lt;/font&gt;</rr:ObjectivePrimaryTextBlock>
  <rr:ObjectiveSecondaryTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;secondarily, capital appreciation. &lt;/font&gt;</rr:ObjectiveSecondaryTextBlock>
  <rr:ExpenseHeading contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: sans-serif; color: rgb(0, 79, 124); font-size: 9.5pt; "&gt;&lt;b&gt;Fees and Expenses &lt;/b&gt;&lt;/font&gt;</rr:ExpenseHeading>
  <rr:ExpenseNarrativeTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;This table describes the fees and expenses that you may pay if you buy and hold shares of the fund. &lt;/font&gt;</rr:ExpenseNarrativeTextBlock>
  <rr:OperatingExpensesCaption contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style=" FONT-STYLE: italic; FONT-FAMILY: Sans-Serif; COLOR: #004f7c; FONT-SIZE: 8pt;"&gt;&lt;b&gt;&lt;i&gt;Fees and Expenses of the Fund&lt;/i&gt;&lt;/b&gt;&lt;/font&gt;&lt;br/&gt;&lt;center&gt;&lt;font style=" FONT-STYLE: italic; FONT-FAMILY: Sans-Serif; COLOR: #004f7c; FONT-SIZE: 8pt;"&gt;&lt;b&gt;Annual fund operating expenses&lt;br/&gt; (expenses that you pay each year as a&lt;br/&gt; percentage of the value of your investment)&lt;/b&gt;&lt;/font&gt;&lt;/center&gt;</rr:OperatingExpensesCaption>
  <rr:PortfolioTurnoverHeading contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="FONT-FAMILY: Sans-Serif; FONT-SIZE: 9.5pt;"&gt;&lt;b&gt;Portfolio Turnover&lt;/b&gt;&lt;/font&gt;</rr:PortfolioTurnoverHeading>
  <rr:PortfolioTurnoverTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;The fund pays transaction costs, such as commissions, when it buys and sells securities (or &amp;#8220;turns over&amp;#8221; its portfolio). A higher portfolio turnover rate may indicate higher transaction costs and may result in higher taxes when fund shares are held in a taxable account. These costs, which are not reflected in annual fund operating expenses or in the example, affect the fund&amp;#8217;s performance.&lt;/font&gt;</rr:PortfolioTurnoverTextBlock>
  <rr:OtherExpensesNewFundBasedOnEstimates contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;Other expenses are estimated for the current fiscal year.&lt;/font&gt;</rr:OtherExpensesNewFundBasedOnEstimates>
  <rr:ExpenseExampleHeading contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="FONT-FAMILY: Sans-Serif; FONT-SIZE: 9.5pt; FONT-WEIGHT: bold"&gt;Example&lt;/font&gt;</rr:ExpenseExampleHeading>
  <rr:ExpenseExampleNarrativeTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;This example is intended to help you compare the cost of investing in the fund with the cost of investing in other mutual funds. The example assumes that you invest $10,000 in the fund for the time periods indicated and then redeem all of your shares at the end of those periods. The example also assumes that your investment has a 5% return each year and that the fund&amp;#8217;s operating expenses remain the same. Although your actual costs may be higher or lower, based on these assumptions your costs would be:&lt;/font&gt;</rr:ExpenseExampleNarrativeTextBlock>
  <rr:StrategyHeading contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: sans-serif; color: rgb(0, 79, 124); font-size: 9.5pt; "&gt;&lt;b&gt;Investments, Risks, and Performance &lt;/b&gt;&lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="FONT-FAMILY: Sans-Serif; FONT-SIZE: 9.5pt;"&gt;&lt;b&gt;Principal Investment Strategies &lt;/b&gt;&lt;/font&gt;</rr:StrategyHeading>
  <rr:RiskHeading contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="FONT-FAMILY: Sans-Serif; FONT-SIZE: 9.5pt;"&gt;&lt;b&gt;Principal Risks &lt;/b&gt;&lt;/font&gt;</rr:RiskHeading>
  <rr:RiskLoseMoney contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;The fund&amp;#8217;s share price fluctuates, which means you could lose money by investing in the fund. &lt;/font&gt;</rr:RiskLoseMoney>
  <rr:BarChartAndPerformanceTableHeading contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="FONT-FAMILY: Sans-Serif; FONT-SIZE: 9.5pt; FONT-WEIGHT: bold"&gt;Performance&lt;/font&gt;</rr:BarChartAndPerformanceTableHeading>
  <rr:PerformanceNarrativeTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;Because the fund commenced operations in 2012, there is no historical performance information shown here. Performance history will be presented after the fund has been in operation for one full calendar year.&lt;/font&gt;</rr:PerformanceNarrativeTextBlock>
  <rr:PerformanceOneYearOrLess contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;Because the fund commenced operations in 2012, there is no historical performance information shown here. Performance history will be presented after the fund has been in operation for one full calendar year.&lt;/font&gt;</rr:PerformanceOneYearOrLess>
  <rr:PerformanceAvailabilityPhone contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;1-800-638-8790&lt;/font&gt;</rr:PerformanceAvailabilityPhone>
  <rr:PerformanceTableClosingTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;Current performance information may be obtained by calling 1-800-638-8790.&lt;/font&gt;</rr:PerformanceTableClosingTextBlock>
  <rr:StrategyNarrativeTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;The fund will normally invest at least 80% of its net assets (including any borrowings for investment purposes) in floating rate loans and floating rate debt securities.&lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="font-family: serif; font-size: 10pt; "&gt;Floating rate loans represent amounts borrowed by companies or other entities from banks and other lenders. In many cases, they are issued in connection with recapitalizations, acquisitions, leveraged buyouts, and refinancings. Most, if not all, of the loans in which the fund invests are rated below investment-grade (BB and lower, or an equivalent rating) or are not rated by a major credit rating agency. The loans in which the fund invests are often referred to as &amp;#8220;leveraged loans&amp;#8221; because the borrowing companies have significantly more debt than equity.&lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="font-family: serif; font-size: 10pt; "&gt;The loans held by the fund may be senior or subordinate obligations of the borrower, although the fund normally invests the majority of its assets in senior floating rate loans. In the event of bankruptcy, holders of senior floating rate loans are typically paid (to the extent assets are available) before certain other creditors of the borrower (e.g., bondholders and stockholders). Holders of subordinate loans may be paid after more senior bondholders. Loans may or may not be secured by collateral. There is no limit on the fund&amp;#8217;s investments in unsecured loans or in companies involved in bankruptcy proceedings, reorganizations, or financial restructurings.&lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="font-family: serif; font-size: 10pt; "&gt;Floating rate loans have interest rates that reset periodically (typically quarterly or monthly). The interest rates on floating rate loans are generally based on a percentage above LIBOR (the London Interbank Offered Rate), a U.S. bank&amp;#8217;s prime or base rate, the overnight federal funds rate, or another rate. Floating rate loans may be structured and administered by a financial institution that acts as the agent of the lenders participating in the floating rate loan. The fund may acquire floating rate loans directly from a lender or through the agent, as an assignment from another lender who holds a floating rate loan, or as a participation interest in another lender&amp;#8217;s floating rate loan or portion thereof. &lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="font-family: serif; font-size: 10pt; "&gt;In buying and selling loans, the fund relies on its fundamental analysis of each company and the company&amp;#8217;s ability to pay principal and interest in light of its current financial condition, its industry position, and economic and market conditions. The fund may purchase other floating rate debt instruments with credit and interest rate characteristics similar to the floating rate loans that it purchases.&lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="font-family: serif; font-size: 10pt; "&gt;In addition to the fund&amp;#8217;s investments in loans, the fund may invest in a variety of debt securities, such as government and agency debt obligations, and investment-grade and high yield corporate bonds. High yield bonds, also known as &amp;#8220;junk&amp;#8221; bonds, are rated below investment grade and should be considered speculative. They generally provide high income in an effort to compensate investors for their higher risk of default, which is the failure to make required interest or principal payments. High yield bond issuers include small or relatively new companies lacking the history or capital to merit investment-grade status, former blue chip companies downgraded because of financial problems, companies electing to borrow heavily to finance or avoid a takeover or buyout, and firms with heavy debt loads. The fund may invest up to 20% of its net assets in fixed rate debt securities. &lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="font-family: serif; font-size: 10pt; "&gt;The fund has considerable flexibility in seeking higher yields. There are no maturity restrictions, so the fund can purchase longer-term loans and bonds, which tend to have higher yields (but are more volatile) than shorter-term loans and bonds. However, the fund&amp;#8217;s weighted average maturity generally is expected to be in the 4- to 8-year range, although it may be above or below that range depending on market conditions.&lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="font-family: serif; font-size: 10pt; "&gt;While most assets will typically be invested in U.S. dollar-denominated floating rate loans and debt securities, the fund may also invest in foreign loans and securities in keeping with the fund&amp;#8217;s objective. While there is no limit on holdings that are issued by foreign issuers, including issuers in emerging markets, the fund may only invest up to 20% of its total assets in non-U.S. dollar-denominated loans and debt securities. &lt;/font&gt;&lt;br/&gt;&lt;br/&gt;&lt;font style="font-family: serif; font-size: 10pt; "&gt;The fund may sell holdings for a variety of reasons, such as to adjust the portfolio&amp;#8217;s average maturity or credit quality, to shift assets into and out of higher-yielding loans or securities, or to reduce its exposure to certain loans or securities.&lt;/font&gt;</rr:StrategyNarrativeTextBlock>
  <rr:RiskNarrativeTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;font style="font-family: serif; font-size: 10pt; "&gt;As with any mutual fund, there is no guarantee that the fund will achieve its objective. The fund&amp;#8217;s share price fluctuates, which means you could lose money by investing in the fund. The fund is exposed to interest rate risk like more traditional bond funds, but credit and liquidity risks tend to be more important. The principal risks of investing in this fund are summarized as follows:&lt;br/&gt;&lt;br/&gt;&lt;b&gt;&lt;i&gt;Active management risk &lt;/i&gt;&lt;/b&gt;The fund is subject to the risk that the investment adviser&amp;#8217;s judgments about the attractiveness, value, or potential appreciation of the fund&amp;#8217;s investments may prove to be incorrect. If the securities selected and strategies employed by the fund fail to produce the intended results, the fund could underperform other funds with similar objectives and investment strategies.&lt;br/&gt;&lt;br/&gt;&lt;i&gt;&lt;b&gt;Credit risk &lt;/b&gt;&lt;/i&gt;This is the risk that a loan borrower or issuer of a debt security could suffer an adverse change in financial condition that results in a payment default, inability to meet a financial obligation, or the downgrade of a fund holding. The fund&amp;#8217;s overall credit risk is increased to the extent it invests in loans not secured by collateral or if it purchases a participation interest in a loan.&lt;br/&gt;&lt;br/&gt;Because a significant portion of the fund&amp;#8217;s investments may be rated below investment-grade (also known as &amp;#8220;junk&amp;#8221; securities), the fund is exposed to greater volatility than if it invested mainly in investment-grade bonds and loans. High yield bond and loan issuers are more likely to suffer an adverse change in financial condition that would result in the inability to meet a financial obligation. Accordingly, securities and loans involving such companies carry a higher risk of default and should be considered speculative.&lt;br/&gt;&lt;br/&gt;&lt;i&gt;&lt;b&gt;Impairment of collateral risk &lt;/b&gt;&lt;/i&gt; This is the risk that the value of collateral securing a floating rate loan could decline, be insufficient to satisfy the loan obligation, or be difficult to liquidate. The fund&amp;#8217;s access to the collateral could be limited by bankruptcy or by the type of loan it purchases. As a result, a collateralized senior loan may not be fully collateralized and can decline significantly in value.&lt;br/&gt;&lt;br/&gt;&lt;i&gt;&lt;b&gt;Interest rate risk&lt;/b&gt;&lt;/i&gt;  This is the risk that a rise in interest rates will cause the price of a fixed rate debt security to fall. Generally, securities with longer maturities and funds with longer weighted average maturities carry greater interest rate risk. Because interest payments on the fund&amp;#8217;s floating rate investments are typically based on a spread over another interest rate, falling interest rates will result in less income for the fund, but will not typically result in the price volatility that a fixed rate holding could experience. &lt;br/&gt;&lt;br/&gt;&lt;i&gt;&lt;b&gt;Prepayment risk &lt;/b&gt;&lt;/i&gt;This is the risk that the principal on a loan or debt security will be prepaid prior to its maturity, reducing the potential for price gains. The rate of prepayments tends to increase as interest rates fall.&lt;br /&gt;&lt;br /&gt;&lt;i&gt;&lt;b&gt;Liquidity risk  &lt;/b&gt;&lt;/i&gt;This is the risk that the fund may not be able to sell a holding in a timely manner at a desired price. Floating rate loans may not have an active trading market and often have contractual restrictions on resale, which can delay the sale and adversely impact the sale price.&lt;br/&gt;&lt;br/&gt;&lt;i&gt;&lt;b&gt;Senior loans risk &lt;/b&gt;&lt;/i&gt;Senior loans are subject to the risk that a court could subordinate a senior loan, which typically holds the most senior position in the issuer&amp;#8217;s capital structure, to presently existing or future indebtedness or take other action detrimental to the holders of senior loans.&lt;br/&gt;&lt;br/&gt;&lt;i&gt;&lt;b&gt;Foreign investing risk&lt;/b&gt;&lt;/i&gt;  This is the risk that the fund&amp;#8217;s investments in foreign securities may be adversely affected by political and economic conditions overseas, reduced liquidity, or decreases in foreign currency values relative to the U.S. dollar. These risks are heightened for the fund&amp;#8217;s investments in emerging markets.&lt;/font&gt;</rr:RiskNarrativeTextBlock>
  <rr:ManagementFeesOverAssets decimals="4" contextRef="Duration_02Jul2011_01Jul2012S000036338_MemberC000111137_Member" unitRef="pure">0</rr:ManagementFeesOverAssets>
  <rr:OtherExpensesOverAssets id="Item_2" decimals="4" contextRef="Duration_02Jul2011_01Jul2012S000036338_MemberC000111137_Member" unitRef="pure">0.0001</rr:OtherExpensesOverAssets>
  <rr:ExpensesOverAssets decimals="4" contextRef="Duration_02Jul2011_01Jul2012S000036338_MemberC000111137_Member" unitRef="pure">0.0001</rr:ExpensesOverAssets>
  <rr:ExpenseExampleYear01 decimals="INF" contextRef="Duration_02Jul2011_01Jul2012S000036338_MemberC000111137_Member" unitRef="USD">1</rr:ExpenseExampleYear01>
  <rr:ExpenseExampleYear03 decimals="INF" contextRef="Duration_02Jul2011_01Jul2012S000036338_MemberC000111137_Member" unitRef="USD">3</rr:ExpenseExampleYear03>
  <rr:ProspectusDate contextRef="Duration_02Jul2011_01Jul2012">2012-07-01</rr:ProspectusDate>
  <rr:AnnualFundOperatingExpensesTableTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;div style="display:none"&gt;~ http://troweprice.com/role/ScheduleAnnualFundOperatingExpensesT.RowePriceFloatingRateMulti-SectorAccountPortfolio column period compact * ~&lt;/div&gt;

</rr:AnnualFundOperatingExpensesTableTextBlock>
  <rr:ExpenseExampleWithRedemptionTableTextBlock contextRef="Duration_02Jul2011_01Jul2012S000036338_Member">&lt;div style="display:none"&gt;~ http://troweprice.com/role/ScheduleExpenseExampleTransposedT.RowePriceFloatingRateMulti-SectorAccountPortfolio column period compact * ~&lt;/div&gt;

</rr:ExpenseExampleWithRedemptionTableTextBlock>
  <link:footnoteLink xlink:type="extended" xlink:role="http://www.xbrl.org/2003/role/link">
    <link:loc xlink:type="locator" xlink:href="#Item_2" xlink:label="OtherExpensesOverAssets" />
    <link:footnote xlink:type="resource" xlink:label="footnote_OtherExpensesOverAssets" xlink:role="http://www.xbrl.org/2003/role/footnote" xml:lang="en-US" id="footnote_OtherExpensesOverAssets">Other expenses are estimated for the current fiscal year. T. Rowe Price Associates, Inc. is required to bear any of the fund's custody fees that exceed 0.01% of the fund's average net assets as well as all of the fund's other operating expenses (except for interest and borrowing expenses, taxes, brokerage fees and commissions, and non-recurring extraordinary expenses). Expenses paid under this agreement are not subject to reimbursement to T. Rowe Price Associates, Inc. by the fund and the arrangement may only be modified or terminated upon approval by the fund's shareholders and Board of Directors. </link:footnote>
    <link:footnoteArc xlink:type="arc" xlink:arcrole="http://www.xbrl.org/2003/arcrole/fact-footnote" xlink:from="OtherExpensesOverAssets" xlink:to="footnote_OtherExpensesOverAssets" />
  </link:footnoteLink>
</xbrl>
