Exhibit 8.1

Subsidiaries of Navios Maritime Acquisition Corporation

Navios Maritime Acquisition Corporation and Subsidiaries:	Nature	Country of Incorporation
Company Name
Aegean Sea Maritime Holdings Inc.	Sub-Holding Company	Marshall Is.
Amorgos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Andros Shipping Corporation	Vessel-Owning Company	Marshall Is.
Antikithira Shipping Corporation	Vessel-Owning Company	Marshall Is.
Antiparos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Amindra Navigation Co.	Sub-Holding Company	Marshall Is.
Crete Shipping Corporation	Vessel-Owning Company	Marshall Is.
Folegandros Shipping Corporation	Vessel-Owning Company	Marshall Is.
Ikaria Shipping Corporation	Vessel-Owning Compan)	Marshall Is.
Ios Shipping Corporation	Vessel-Owning Company	Cayman Is.
Kithira Shipping Corporation	Vessel-Owning Company	Marshall Is.
Kos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Mytilene Shipping Corporation	Vessel-Owning Company	Marshall Is.
Navios Maritime Midstream Partners GP LLC	Holding Company	Marshall Is.
Navios Acquisition Finance (U.S.) Inc.	Co-Issuer	Delaware
Rhodes Shipping Corporation	Vessel-Owning Company	Marshall Is.
Serifos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Shinyo Loyalty Limited	Former Vessel-Owning Company	Hong Kong
Shinyo Navigator Limited	Former Vessel-Owning Company	Hong Kong
Sifnos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Skiathos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Skopelos Shipping Corporation	Vessel-Owning Company	Cayman Is.
Syros Shipping Corporation	Vessel-Owning Company	Marshall Is.
Thera Shipping Corporation	Vessel-Owning Company	Marshall Is.

Tinos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Oinousses Shipping Corporation	Vessel-Owning Company	Marshall Is.
Psara Shipping Corporation	Vessel-Owning Company	Marshall Is.
Antipsara Shipping Corporation	Vessel-Owning Company	Marshall Is.
Samothrace Shipping Corporation	Vessel-Owning Company	Marshall Is.
Thasos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Limnos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Skyros Shipping Corporation	Vessel-Owning Company	Marshall Is.
Alonnisos Shipping Corporation	Former Vessel-Owning Company	Marshall Is.
Makronisos Shipping Corporation	Former Vessel-Owning Company	Marshall Is.
Iraklia Shipping Corporation	Vessel-Owning Company	Marshall Is.
Paxos Shipping Corporation	Former Vessel-Owning Company	Marshall Is.
Antipaxos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Donoussa Shipping Corporation	Former Vessel-Owning Company	Marshall Is.
Schinousa Shipping Corporation	Former Vessel-Owning Company	Marshall Is.
Navios Acquisition Europe Finance Inc	Sub-Holding Company	Marshall Is.
Kerkyra Shipping Corporation	Vessel-Owning Company	Marshall Is.
Lefkada Shipping Corporation	Vessel-Owning Company	Marshall Is.
Zakynthos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Leros Shipping Corporation	Vessel-Owning Company	Marshall Is.
Kimolos Shipping Corporation	Former Vessel-Owning Company	Marshall Is.
Samos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Tilos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Delos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Agistri Shipping Corporation	Operating Subsidiary	Malta
Olivia Enterprises Corp.	Vessel-Owning Company	Marshall Is.
Cyrus Investments Corp.	Vessel-Owning Company	Marshall Is.
Doxa International Corp.	Vessel-Owning Company	Marshall Is.

Navios Maritime Midstream Operating LLC	Sub-Holding Company	Marshall Is.
Navios Maritime Midstream Partners L.P.	Sub-Holding Company	Marshall Is.
Navios Maritime Midstream Partners Finance (US) Inc.	Co-borrower	Delaware
Shinyo Kannika Limited	Former Vessel-Owning Company	Hong Kong
Shinyo Ocean Limited	Former Vessel-Owning Company	Hong Kong
Shinyo Saowalak Limited	Vessel-Owning Company	British Virgin Is.
Shinyo Kieran Limited	Vessel-Owning Company	British Virgin Is.
Shinyo Dream Limited	Former Vessel-Owning Company	Hong Kong
Sikinos Shipping Corporation	Vessel-Owning Company	Marshall Is.
Alkmene Shipping Corporation	Vessel-Owning Company	Marshall Is.
Persephone Shipping Corporation	Vessel-Owning Company	Marshall Is.
Rhea Shipping Corporation	Vessel-Owning Company	Marshall Is.
Aphrodite Shipping Corporation	Vessel-Owning Company	Marshall Is.
Dione Shipping Corporation	Vessel-Owning Company	Marshall Is.