EXHIBIT 10.1

SIXTH SUPPLEMENTAL INDENTURE (this “Sixth Supplemental Indenture”), dated as of November 17, 2015, among Navios Maritime Acquisition Corporation, a Marshall Islands corporation, (the “Company”), Navios Acquisition Finance (US) Inc., a Delaware corporation (together with the Company, the “Co-Issuers”), and Delos Shipping Corporation and Tilos Shipping Corporation, (each a “Guaranteeing Subsidiary”), each a Marshall Islands corporation and an indirect subsidiary of the Company, the other Guarantors (as defined in the Indenture referred to herein) and Wells Fargo Bank, National Association, as trustee (or its permitted successor) under the Indenture referred to below (the “Trustee”) and as collateral trustee (or its permitted successor) under the Indenture referred to below (the “Collateral Trustee”).

WITNESSETH

WHEREAS, the Co-Issuers and the Guarantors have heretofore executed and delivered to the Trustee an indenture (the “Indenture”), dated as of November 13, 2013 providing for the issuance of 8.125% First Priority Ship Mortgage Notes due 2021 (the “Notes”);

WHEREAS, the Indenture provides that under certain circumstances each Guaranteeing Subsidiary shall execute and deliver to the Trustee a supplemental indenture pursuant to which each Guaranteeing Subsidiary shall unconditionally guarantee all of the Co-Issuers’ obligations under the Notes and the Indenture on the terms and conditions set forth herein (the “Note Guarantee”); and

WHEREAS, pursuant to Section 9.01 of the Indenture, the Trustee is authorized to execute and deliver this Sixth Supplemental Indenture.

NOW, THEREFORE, in consideration of the foregoing and for other good and valuable consideration, the receipt of which is hereby acknowledged, each Guaranteeing Subsidiary and the Trustee mutually covenant and agree for the equal and ratable benefit of the Holders of the Notes as follows:

1. CAPITALIZED TERMS. Capitalized terms used herein without definition shall have the meanings assigned to them in the Indenture.

2. AGREEMENT TO GUARANTEE. Each Guaranteeing Subsidiary hereby agrees to provide an unconditional Guarantee, on and subject to the terms, conditions and limitations set forth in the Notation of Guarantee and in the Indenture, including, but not limited, to Article Ten thereof.

3. NEW YORK LAW TO GOVERN. THIS SIXTH SUPPLEMENTAL INDENTURE SHALL BE GOVERNED BY AND CONSTRUED IN ACCORDANCE WITH THE LAWS OF THE STATE OF NEW YORK, WITHOUT GIVING EFFECT TO CONFLICTS OF LAW PRINCIPLES TO THE EXTENT THAT THE APPLICATION OF THE LAWS OF ANOTHER JURISDICTION WOULD BE REQUIRED THEREBY.

5. COUNTERPARTS. The parties may sign any number of copies of this Sixth Supplemental Indenture. Each signed copy shall be an original, but all of them together represent the same agreement.

6. EFFECT OF HEADINGS. The Section headings herein are for convenience only and shall not affect the construction hereof.

7. THE TRUSTEE. The Trustee shall not be responsible in any manner whatsoever for or in respect of the validity or sufficiency of this Sixth Supplemental Indenture or for or in respect of the recitals contained herein, all of which recitals are made solely by each Guaranteeing Subsidiary and the Co-Issuers.

IN WITNESS WHEREOF, the parties hereto have caused this Sixth Supplemental Indenture to be duly executed and attested, all as of the date first above written.

	DELOS SHIPPING CORPORATION TILOS SHIPPING CORPORATION
		By:	/s/ Alexandros Laios
			Name:	Alexandros Laios
			Title:	Secretary/Director
	NAVIOS MARITIME ACQUISITION CORPORATION
		By:	/s/ Vasiliki Papaefthymiou
			Name:	Vasiliki Papaefthymiou
			Title:	Secretary
	NAVIOS ACQUISITION FINANCE (US) INC.
		By:	/s/ Vasiliki Papaefthymiou
			Name:	Vasiliki Papaefthymiou
			Title:	President/Secretary
NAVIOS ACQUISITION EUROPE FINANCE INC.
		By:	/s/ Alexandros Laios
		Name:	Alexandros Laios
		Title:	Director/Secretary
	LEROS SHIPPING CORPORATION KIMOLOS SHIPPING CORPORATION LEFKADA SHIPPING CORPORATION KERKYRA SHIPPING CORPORATION ZAKYNTHOS SHIPPING CORPORATION DONOUSSA SHIPPING CORPORATION SCHINOUSA SHIPPING CORPORATION SIKINOS SHIPPING CORPORATION LIMNOS SHIPPING CORPORATION SKYROS SHIPPING CORPORATION

ALONNISOS SHIPPING CORPORATION
MAKRONISOS SHIPPING CORPORATION
IRAKLIA SHIPPING CORPORATION
THASOS SHIPPING CORPORATION
SAMOTHRACE SHIPPING CORPORATION
PAXOS SHIPPING CORPORATION
ANTIPAXOS SHIPPING CORPORATION
OINOUSSES SHIPPING CORPORATION
ANTIPSARA SHIPPING CORPORATION
PSARA SHIPPING CORPORATION
KITHIRA SHIPPING CORPORATION
ANTIKITHIRA SHIPPING CORPORATION
AMINDRA NAVIGATION CO.
SERIFOS SHIPPING CORPORATION
FOLEGANDROS SHIPPING CORPORATION
	By:	/s/ Anna Kalathakis
	Name:	Anna Kalathakis
	Title:	Treasurer/Director
SHINYO DREAM LIMITED
SHINYO KANNIKA LIMITED
SHINYO LOYALTY LIMITED
SHINYO NAVIGATOR LIMITED
SHINYO OCEAN LIMITED
SHINYO SAOWALAK LIMITED SHINYO KIERAN LIMITED
By:	/s/ Alexandros Laios
	Name:	Alexandros Laios
	Title:	Director
AEGEAN SEA MARITIME HOLDINGS INC. THERA SHIPPING CORPORATION TINOS SHIPPING CORPORATION AMORGOS SHIPPING CORPORATION ANDROS SHIPPING CORPORATION ANTIPAROS SHIPPING CORPORATION CRETE SHIPPING CORPORATION IKARIA SHIPPING CORPORATION IOS SHIPPING CORPORATION KOS SHIPPING CORPORATION MYTILENE SHIPPING CORPORATION RHODES SHIPPING CORPORATION SIFNOS SHIPPING CORPORATION

SKIATHOS SHIPPING CORPORATION SKOPELOS SHIPPING CORPORATION SYROS SHIPPING CORPORATION
By:	/s/ George Achniotis
	Name:	George Achniotis
	Title:	President/Director
WELLS FARGO BANK, NATIONAL ASSOCIATION, as Trustee
	By:	/s/ Yana Kislenko
		Name:	Yana Kislenko
		Title:	Vice President
WELLS FARGO BANK, NATIONAL ASSOCIATION, as Collateral Trustee
	By:	/s/ Yana Kislenko
		Name:	Yana Kislenko
		Title:	Vice President