<?xml version="1.0" encoding="us-ascii"?>
<!-- EDGAR Online I-Metrix Xcelerate Risk Return XBRL Instance Document, based on XBRL 2.1  http://www.edgar-online.com/ -->
<!-- Created (UTC): 10/5/2012 7:34:11 PM -->
<!-- XBRL File Set ID: bcd5bc52-bb73-4b5a-865d-3653e42c04ec -->
<!-- App Version: 3.0.0.183, 6.1.7601.65536, 14.0 -->
<!-- Copyright (c) 2005-2012 EDGAR Online, Inc. All Rights Reserved. -->
<xbrl xmlns="http://www.xbrl.org/2003/instance" xmlns:link="http://www.xbrl.org/2003/linkbase" xmlns:ixt="http://www.xbrl.org/2008/inlineXBRL/transformation" xmlns:xbrldt="http://xbrl.org/2005/xbrldt" xmlns:rr="http://xbrl.sec.gov/rr/2010-02-28" xmlns:dei="http://xbrl.us/dei/2009-01-31" xmlns:xbrldi="http://xbrl.org/2006/xbrldi" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:iso4217="http://www.xbrl.org/2003/iso4217" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xbrli="http://www.xbrl.org/2003/instance" xmlns:ck0001432353="http://www.globalxfunds.com/20121003">
  <link:schemaRef xlink:href="ck0001432353-20121003.xsd" xlink:type="simple" />
  <context id="DocumentContext" xmlns="http://www.xbrl.org/2003/instance">
    <entity>
      <identifier scheme="http://www.sec.gov/CIK">0001432353</identifier>
    </entity>
    <period>
      <startDate>2012-10-03</startDate>
      <endDate>2012-10-03</endDate>
    </period>
  </context>
  <context id="S000024773member_C000073664member" xmlns="http://www.xbrl.org/2003/instance">
    <entity>
      <identifier scheme="http://www.sec.gov/CIK">0001432353</identifier>
      <segment>
        <xbrldi:explicitMember dimension="dei:LegalEntityAxis" xmlns:xbrldi="http://xbrl.org/2006/xbrldi">ck0001432353:S000024773Member</xbrldi:explicitMember>
        <xbrldi:explicitMember dimension="rr:ProspectusShareClassAxis" xmlns:xbrldi="http://xbrl.org/2006/xbrldi">ck0001432353:C000073664Member</xbrldi:explicitMember>
      </segment>
    </entity>
    <period>
      <startDate>2012-10-03</startDate>
      <endDate>2012-10-03</endDate>
    </period>
  </context>
  <context id="CTXT_P0001S000024773member_S000024773member" xmlns="http://www.xbrl.org/2003/instance">
    <entity>
      <identifier scheme="http://www.sec.gov/CIK">0001432353</identifier>
      <segment>
        <xbrldi:explicitMember dimension="dei:DocumentInformationDocumentAxis" xmlns:xbrldi="http://xbrl.org/2006/xbrldi">ck0001432353:P0001-S000024773Member</xbrldi:explicitMember>
        <xbrldi:explicitMember dimension="dei:LegalEntityAxis" xmlns:xbrldi="http://xbrl.org/2006/xbrldi">ck0001432353:S000024773Member</xbrldi:explicitMember>
      </segment>
    </entity>
    <period>
      <startDate>2012-10-03</startDate>
      <endDate>2012-10-03</endDate>
    </period>
  </context>
  <dei:DocumentType contextRef="DocumentContext">Other</dei:DocumentType>
  <dei:DocumentPeriodEndDate contextRef="DocumentContext">2012-10-03</dei:DocumentPeriodEndDate>
  <dei:EntityRegistrantName contextRef="DocumentContext">Global X Funds</dei:EntityRegistrantName>
  <dei:EntityCentralIndexKey contextRef="DocumentContext">0001432353</dei:EntityCentralIndexKey>
  <dei:AmendmentFlag contextRef="DocumentContext">false</dei:AmendmentFlag>
  <dei:DocumentCreationDate contextRef="DocumentContext">2012-10-03</dei:DocumentCreationDate>
  <dei:DocumentEffectiveDate contextRef="DocumentContext">2012-10-03</dei:DocumentEffectiveDate>
  <rr:ProspectusDate contextRef="DocumentContext">2012-03-01</rr:ProspectusDate>
  <rr:RiskReturnHeading contextRef="CTXT_P0001S000024773member_S000024773member">Global X FTSE Colombia 20 ETF</rr:RiskReturnHeading>
  <ck0001432353:SupplementTextBlock contextRef="CTXT_P0001S000024773member_S000024773member">&lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt;line-height: .05pt;text-autospace:none"&gt;&lt;font style=&apos;font-size:12.0pt; font-family:"Times New Roman","serif"&apos;&gt;&amp;nbsp;&lt;/font&gt;&lt;/p&gt;  &lt;p class="MsoNormal" align="center" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt; text-align:center;line-height:normal;text-autospace:none"&gt;&lt;b&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;GLOBAL X FUNDS&lt;/font&gt;&lt;/b&gt;&lt;/p&gt;  &lt;p class="MsoNormal" align="center" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt; text-align:center;line-height:normal;text-autospace:none"&gt;&lt;b&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;&amp;nbsp;&lt;/font&gt;&lt;/b&gt;&lt;/p&gt;  &lt;p class="MsoNormal" align="center" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt; text-align:center;line-height:normal;text-autospace:none"&gt;&lt;b&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;SUPPLEMENT DATED OCTOBER 3, 2012&lt;/font&gt;&lt;/b&gt;&lt;/p&gt;  &lt;p class="MsoNormal" align="center" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt; text-align:center;line-height:normal;text-autospace:none"&gt;&lt;b&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;TO THE PROSPECTUS AND SUMMARY PROSPECTUS DATED MARCH 1, 2012&lt;/font&gt;&lt;/b&gt;&lt;/p&gt;  &lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt;line-height: normal;text-autospace:none"&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;&amp;nbsp;&lt;/font&gt;&lt;/p&gt;  &lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt;text-align: justify;line-height:normal;text-autospace:none"&gt;&lt;font style=&apos;font-size:10.0pt; font-family:"Times New Roman","serif"&apos;&gt;This Supplement updates certain information contained in the above-dated Prospectus and Summary Prospectus for Global X Funds (&#8220;Trust&#8221;) regarding Global X FTSE Colombia 20 ETF (&#8220;Fund&#8221;), a series of the Trust.&lt;/font&gt;&lt;/p&gt;  &lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;margin-bottom:10.0pt;line-height:115%;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;"&gt;&amp;nbsp;&lt;/p&gt;</ck0001432353:SupplementTextBlock>
  <rr:StrategyHeading contextRef="CTXT_P0001S000024773member_S000024773member">Effective immediately, the following paragraph replaces the paragraph under &#8220;Principal Investment Strategies&#8221; on page 7 of the Prospectus and page 2 of the Summary Prospectus:  &amp;nbsp;</rr:StrategyHeading>
  <rr:StrategyNarrativeTextBlock contextRef="CTXT_P0001S000024773member_S000024773member">&lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt;text-align: justify;line-height:normal;text-autospace:none"&gt;&lt;font style=&apos;font-size:10.0pt; font-family:"Times New Roman","serif"&apos;&gt;The Fund invests its total assets in securities of companies that are listed on the Colombian Stock Exchange as well as cash and cash equivalents. The Fund also invests at least 80% of its total assets in the securities of the Underlying Index and in ADRs and GDRs based on the securities in the Underlying Index. The Fund&#8217;s investment policies described above are non-fundamental and require 60 days&#8217; prior written notice to shareholders before they can be changed. The Fund may lend securities representing up to one-third of the value of the Fund&#8217;s total assets (including the value of the collateral received).&lt;/font&gt;&lt;/p&gt;  &lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;margin-bottom:10.0pt;line-height:115%;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;"&gt;&amp;nbsp;&lt;/p&gt;</rr:StrategyNarrativeTextBlock>
  <ck0001432353:SupplementClosingTextBlock contextRef="CTXT_P0001S000024773member_S000024773member">&lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt;text-align: justify;line-height:normal;text-autospace:none"&gt;&lt;font style=&apos;font-size:12.0pt; font-family:"Times New Roman","serif"&apos;&gt;&amp;nbsp;&lt;/font&gt;&lt;/p&gt;  &lt;p class="MsoNormal" align="center" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt; text-align:center;line-height:normal;text-autospace:none"&gt;&lt;b&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;&amp;nbsp;&lt;/font&gt;&lt;/b&gt;&lt;/p&gt;  &lt;p class="MsoNormal" align="center" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt; text-align:center;line-height:normal;text-autospace:none"&gt;&lt;b&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;PLEASE RETAIN THIS SUPPLEMENT FOR FUTURE REFERENCE&lt;/font&gt;&lt;/b&gt;&lt;/p&gt;  &lt;p class="MsoNormal" align="center" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt; text-align:center;line-height:normal;text-autospace:none"&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;&amp;nbsp;&lt;/font&gt;&lt;/p&gt;  &lt;p class="MsoNormal" align="center" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt; text-align:center;line-height:normal;text-autospace:none"&gt;&lt;font style=&apos;font-size:10.0pt;font-family:"Times New Roman","serif"&apos;&gt;&amp;nbsp;&lt;/font&gt;&lt;/p&gt;  &lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;margin-bottom:0in;margin-bottom:.0001pt;line-height: 12.0pt;text-autospace:none"&gt;&lt;font style=&apos;font-size:12.0pt;font-family:"Times New Roman","serif"&apos;&gt;&amp;nbsp;&lt;/font&gt;&lt;/p&gt;  &lt;p class="MsoNormal" style="margin-top:0in;margin-right:0in;margin-left:0in;margin-bottom:10.0pt;line-height:115%;font-size:11.0pt;font-family:&apos;calibri&apos;,&apos;sans-serif&apos;;"&gt;&amp;nbsp;&lt;/p&gt;</ck0001432353:SupplementClosingTextBlock>
  <dei:TradingSymbol contextRef="S000024773member_C000073664member">GXG</dei:TradingSymbol>
  <link:footnoteLink xlink:type="extended" xlink:role="http://www.xbrl.org/2003/role/link">
  </link:footnoteLink>
</xbrl>
