Exhibit 21.1

Subsidiaries of

Apple REIT Six, Inc.

At December 31, 2012

(The state of incorporation or organization of each subsidiary is Virginia, except as noted)

A. Direct Subsidiaries

Apple Fund Management, LLC

Apple Six Hospitality, Inc.

Apple Six Residential, Inc.

Apple Six Ventures, Inc.

Apple Air Holding, LLC (26% ownership)

B. Indirect Subsidiaries (held through direct subsidiaries or other indirect subsidiaries)

Appleseed Interactive, LLC

Apple Six Anchorage, Inc.

Apple Six Anchorage Management, Inc.

Apple Six Foothill Ranch, Inc.

Apple Six Foothill Ranch Services, Inc.

Apple Six Hospitality Management, Inc.

Apple Six Hospitality Ownership, Inc.

Apple Six Hospitality Texas, L.P.

Apple Six NC GP, Inc.

Apple Six NC LP, Inc.

Apple Six North Carolina, L.P.

Apple Six Services I, LLC

Apple Six Services II, LLC

Apple Six Services III, LLC

Apple Six Services AK Holdings, Inc.

Apple Six Services Anchorage I, LLC

Apple Six Services Anchorage II, LLC

Apple Six Services General, Inc.

Apple Six Services Glendale, Inc.

Apple Six Services Hillsboro CY, Inc.

Apple Six Services Huntsville, Inc.

Apple Six Services Limited, Inc.

Apple Six Services Montgomery, Inc.

Apple Six Services Savannah, Inc.

Apple Six Services TN General, Inc.

Apple Six Services TN Limited, Inc.

Apple Six Services Tennessee, L.P.

Apple Six SPE Fort Worth, Inc.

Apple Six SPE Hillsboro CY, Inc.

Apple Six Tennessee, Inc.

Apple Six Texas General, Inc.

Apple Six Texas Limited, Inc.

Blumberg-Dothan Motel II, LLC *

Davis Highway Motel, Inc. **

RI-Lakeland, LLC **

Sunbelt-CAG, LLC *

Sunbelt-CPC, LLC **

Sunbelt-FCF, LLC **

Sunbelt-FOF, LLC **

Sunbelt-IDA, LLC *

Sunbelt-IPF, LLC **

Sunbelt-RCG, LLC *

Sunbelt Hotel Enterprises, Inc. *

Sunbelt Hotels-Florida II, LLC **

Sunbelt-Huntsville II, LLC *

* State of organization is Alabama.

** State of organization is Florida.