<?xml version="1.0" encoding="us-ascii" standalone="no"?>
<!--XBRL document created with Bowne Tagger XBRL Enabler by Bowne Software version 10.0.0.34-->
<!--Based on XBRL 2.1-->
<!--Created on: 5/20/2009 12:43:07 AM-->
<!--Modified on: 11/24/2009 6:09:14 AM-->
<linkbase xmlns="http://www.xbrl.org/2003/linkbase" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:xlink="http://www.w3.org/1999/xlink" xsi:schemaLocation="http://www.xbrl.org/2003/linkbase http://www.xbrl.org/2003/xbrl-linkbase-2003-12-31.xsd" xmlns:xbrldt="http://xbrl.org/2005/xbrldt">
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#StatementOfConditionParenthetical" roleURI="http://spdrgoldshares.com/role/StatementOfConditionParenthetical" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#RecentAccountingPronouncements" roleURI="http://spdrgoldshares.com/role/RecentAccountingPronouncements" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#QuarterlyStatementsOfOperations" roleURI="http://spdrgoldshares.com/role/QuarterlyStatementsOfOperations" />
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#domain-member" arcroleURI="http://xbrl.org/int/dim/arcrole/domain-member" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#StatementsOfOperations" roleURI="http://spdrgoldshares.com/role/StatementsOfOperations" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#StatementsOfCashFlows" roleURI="http://spdrgoldshares.com/role/StatementsOfCashFlows" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#DocumentAndEntityInformation" roleURI="http://spdrgoldshares.com/role/DocumentAndEntityInformation" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#StatementOfChangesInShareholdersEquity" roleURI="http://spdrgoldshares.com/role/StatementOfChangesInShareholdersEquity" />
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#all" arcroleURI="http://xbrl.org/int/dim/arcrole/all" />
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#hypercube-dimension" arcroleURI="http://xbrl.org/int/dim/arcrole/hypercube-dimension" />
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-domain" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-domain" />
  <arcroleRef xlink:type="simple" xlink:href="http://www.xbrl.org/2005/xbrldt-2005.xsd#dimension-default" arcroleURI="http://xbrl.org/int/dim/arcrole/dimension-default" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#Organization" roleURI="http://spdrgoldshares.com/role/Organization" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#SignificantAccountingPolicies" roleURI="http://spdrgoldshares.com/role/SignificantAccountingPolicies" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#InvestmentInGold" roleURI="http://spdrgoldshares.com/role/InvestmentInGold" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#ConcentrationOfRisk" roleURI="http://spdrgoldshares.com/role/ConcentrationOfRisk" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#Indemnification" roleURI="http://spdrgoldshares.com/role/Indemnification" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#RelatedPartiesSponsorTrusteeCustodianAndMarketingAgentFees" roleURI="http://spdrgoldshares.com/role/RelatedPartiesSponsorTrusteeCustodianAndMarketingAgentFees" />
  <roleRef xlink:type="simple" xlink:href="gld-20090930.xsd#BalanceSheets" roleURI="http://spdrgoldshares.com/role/BalanceSheets" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/Organization" xlink:title="0601 - Disclosure - Organization" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/SignificantAccountingPolicies" xlink:title="0602 - Disclosure - Significant accounting policies" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/InvestmentInGold" xlink:title="0603 - Disclosure - Investment in Gold" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/ConcentrationOfRisk" xlink:title="0606 - Disclosure - Concentration of Risk" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/Indemnification" xlink:title="0607 - Disclosure - Indemnification" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/RelatedPartiesSponsorTrusteeCustodianAndMarketingAgentFees" xlink:title="0605 - Disclosure - Related Parties Sponsor, Trustee, Custodian and Marketing Agent Fees" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/BalanceSheets" xlink:title="01 - Statement - Condensed Statements of Condition">
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementLineItems" xlink:label="loc_StatementLineItems" />
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementTable" xlink:label="loc_StatementTable" />
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_StatementLineItems" xlink:to="loc_StatementTable" order="1" xbrldt:contextElement="segment" />
  </definitionLink>
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/StatementsOfOperations" xlink:title="02 - Statement - Condensed Statements of Operations">
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementLineItems" xlink:label="loc_StatementLineItems" />
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementTable" xlink:label="loc_StatementTable" />
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_StatementLineItems" xlink:to="loc_StatementTable" order="1" xbrldt:contextElement="segment" />
  </definitionLink>
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/StatementsOfCashFlows" xlink:title="03 - Statement - Condensed Statements of Cash Flows">
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementLineItems" xlink:label="loc_StatementLineItems" />
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementTable" xlink:label="loc_StatementTable" />
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_StatementLineItems" xlink:to="loc_StatementTable" order="1" xbrldt:contextElement="segment" />
  </definitionLink>
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/DocumentAndEntityInformation" xlink:title="00 - Document - Document and Company Information" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/StatementOfChangesInShareholdersEquity" xlink:title="04 - Statement - Condensed Statement of Changes in Shareholders' Deficit">
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementLineItems" xlink:label="loc_StatementLineItems" />
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementTable" xlink:label="loc_StatementTable" />
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_StatementLineItems" xlink:to="loc_StatementTable" order="1" xbrldt:contextElement="segment" />
  </definitionLink>
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/StatementOfConditionParenthetical" xlink:title="011 - Statement - Condensed Statements of Condition (Footnote Amounts)">
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementLineItems" xlink:label="loc_StatementLineItems" />
    <loc xlink:type="locator" xlink:href="http://taxonomies.xbrl.us/us-gaap/2009/elts/us-gaap-2009-01-31.xsd#us-gaap_StatementTable" xlink:label="loc_StatementTable" />
    <definitionArc xlink:type="arc" xlink:arcrole="http://xbrl.org/int/dim/arcrole/all" xlink:from="loc_StatementLineItems" xlink:to="loc_StatementTable" order="1" xbrldt:contextElement="segment" />
  </definitionLink>
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/RecentAccountingPronouncements" xlink:title="0608 - Disclosure - Recent Accounting Pronouncements" />
  <definitionLink xlink:type="extended" xlink:role="http://spdrgoldshares.com/role/QuarterlyStatementsOfOperations" xlink:title="0604 - Disclosure - Quarterly Statements of Operations (Unaudited)" />
</linkbase>
